秋水仙碱对斑马鱼肝脏和鳃组织SOD和Na^+-K^+-ATPase活性的影响  

Effects of colchicine on the activities of SOD and Na^+-K^+-ATPase in Brachyclanio rerio (Zebrafish)

作　　者：王稼农[1] 黄健[1] 黄仁彬[1] 蒋伟哲[1] 刘华钢[1] 

机构地区：[1]广西医科大学药学院,南宁530021

出　　处：《中国新药杂志》2010年第14期1247-1251,共5页Chinese Journal of New Drugs

基　　金：广西科学研究与技术开发计划项目(桂科合0815007-2-5)

摘　　要：目的:研究不同浓度的秋水仙碱对斑马鱼肝脏和鳃组织超氧化物歧化酶(SOD)和Na+-K+-ATPase活性的影响,为以斑马鱼的酶类作为观测指标进行药物安全性评价提供依据。方法:在预实验的基础上将斑马鱼暴露于秋水仙碱5个浓度组中进行96 h急性毒性试验,计算其半数致死浓度(LC50)。此外,设置3个浓度(0.67,1.70,4.26 mg.L-1),进行21 d慢性毒性试验,期间每7 d测定斑马鱼的肝脏和鳃中的SOD和Na+-K+-ATPase活性。结果:秋水仙碱对斑马鱼的LC50为16.90 mg.L-1。随秋水仙碱浓度增大和染毒时间延长,斑马鱼鳃中SOD和Na+-K+-ATPase活性均显著受到抑制;肝脏SOD活性增加,Na+-K+-AT-Pase活性总体呈现抑制趋势。结论:斑马鱼肝脏和鳃的SOD及Na+-K+-ATPase对毒物敏感,可作为观测指标用于评价药物的毒性。Objectives：To study the effects of different concentrations of colchicine on the activities of SOD and Na^＋-K^＋-ATPase in liver and branchi of Brachyclanio rerio（zebrafish）,and to provide a basis for drug safety for evaluating Brachyclanio rerio＇s enzyme activities as indexes.Methods：Based on the preliminary experiments,Brachyclanio rerio exposed to 5 concentrations of colchicine for 96 h.The median lethal concentration（LC50） was calculated for evaluating acute toxicity.In addition,Brachyclanio rerio exposed to 3 concentrations of colchicine（0.67,1.70 and 4.26 mg·L-1） for 21 days in chronic toxicity test.The activities of SOD and Na^＋-K^＋-ATPase in the liver and branchi of Brachyclanio rerio were measured at intervals of 7 days.Results：LC50 of colchicine was 16.90 mg·L^-1.With increasing colchicine concentrations and exposure time,the activities of SOD and Na^＋-K^＋-ATPase were significantly inhibited in the branchi of Brachyclanio rerio.The SOD activity was inceased but Na^＋-K^＋-ATPase activity tended to decrease in the liver.Conclusions：The SOD and Na^＋-K^＋-ATPase in liver and branchi of Brachyclanio rerio are sensitive to toxicants,and can be used as the index for evaluating drug toxicity.

关 键 词：斑马鱼 秋水仙碱 超氧化物歧化酶 Na^+-K^+-三磷酸腺苷酶 

分 类 号：R965.1[医药卫生—药理学] R994.2[医药卫生—药学]