Amperometric Hydrogen Peroxide Biosensor Based on Multiwall Carbon Nanotubes and Cadmium Sulfide Quantum Dots  

作　　者：ZHANG Jin-lei TAN Xue-cai ZHAO Dan-dan TAN Sheng-wei HUANG Zeng-wei MI Yan HUANG Zai-yin 

机构地区：[1]College of Chemistry and Ecological Engineering, Guangxi University for Nationalities, Nanning 530006, P. R. China [2]Department of Chemistry and Life Science, Baise University, Baise 533000, P. R. China

出　　处：《Chemical Research in Chinese Universities》2010年第4期541-545,共5页高等学校化学研究（英文版）

基　　金：Supported by the Natural Science Foundation of Guangxi Province,China(Nos.0639025 and 0991084);the Support Program for 100 Young and Middle-aged Disciplinary Leaders in Higher Education Institutions of Guangxi Province,China(No. RC20060703005);the Key Laboratory of Development and Application of Forest Chemicals of Guangxi Province,China (No.GXFC08-06);the Education Department of Guangxi Province,China(No.200807MS074);the Innovation Project of Guangxi University fot Nationalities,China(No.gxun-chx2009081)

摘　　要：A novel third-generation hydrogen peroxide（H2O2） biosensor（Hb/CdS/MWNTs/GCE） was fabricated through hemoglobin（Hb） adsorbed onto the mercaptoacetic acid modified CdS QDs/carboxyl multiwall carbon nanotubes＇（MWNTs） films. Cyclic voltammogram of Hb/CdS/MWNTs/GCE showed a pair of well-defined and quasi-reversible redox peaks with a formal potential（E^0） of-0.230 V（vs. Ag/AgCl） in 0.1 mol/L pH=8.0 phosphate buffer solution（PBS）, which was the characteristic of the Hb heme Fe（Ⅲ）/Fe（Ⅱ） redox couple. The biosensor shows an excellent electrocatalytic activity to the reduction of H2O2. The response time of the designed biosensor to H202 at a potential of-0.30 V was less than 2 s and linear relationships were obtained in the concentration ranges of 2.0×10^-6-2.7×10^-3 mol/L and 2.7×10^-3-7.7×10^-3 mol/L with a detection limit of 3.0×10^-7 mol/L（S/N=3）. The apparent Michaelis-Menten constant Km was estimated to be 1.324 mmol/L that illustrated the excellent biological activity of the fixed Hb.A novel third-generation hydrogen peroxide（H2O2） biosensor（Hb/CdS/MWNTs/GCE） was fabricated through hemoglobin（Hb） adsorbed onto the mercaptoacetic acid modified CdS QDs/carboxyl multiwall carbon nanotubes＇（MWNTs） films. Cyclic voltammogram of Hb/CdS/MWNTs/GCE showed a pair of well-defined and quasi-reversible redox peaks with a formal potential（E^0） of-0.230 V（vs. Ag/AgCl） in 0.1 mol/L pH=8.0 phosphate buffer solution（PBS）, which was the characteristic of the Hb heme Fe（Ⅲ）/Fe（Ⅱ） redox couple. The biosensor shows an excellent electrocatalytic activity to the reduction of H2O2. The response time of the designed biosensor to H202 at a potential of-0.30 V was less than 2 s and linear relationships were obtained in the concentration ranges of 2.0×10^-6-2.7×10^-3 mol/L and 2.7×10^-3-7.7×10^-3 mol/L with a detection limit of 3.0×10^-7 mol/L（S/N=3）. The apparent Michaelis-Menten constant Km was estimated to be 1.324 mmol/L that illustrated the excellent biological activity of the fixed Hb.

关 键 词：HEMOGLOBIN CdS quantum dot Multi-wall carbon nanotube BIOSENSOR Direct electrochemistry 

分 类 号：O471.1[理学—半导体物理] TP212.3[理学—物理]