Monitoring photo-thermal response and collagen remodeling of mouse skin with multiphoton microscopy  

作　　者：吴淑莲 李晖 章小曼 

机构地区：[1]Key Laboratory of OptoElectronic Science and Technology for Medicine,Ministry of Education,Fujian Provincial Key Laboratory of Photonic Technology,School of Physics and OptoElectronics Technology,Fujian Normal University

出　　处：《Chinese Optics Letters》2010年第8期784-786,共3页中国光学快报（英文版）

基　　金：supported by the Research Fund for the Doctoral Program of Higher Education(No. 200803940001);the Natural Science Foundation of Fujian Province(No.2010J01323);the National Natural Science Foundation of China(No.60578056)

摘　　要：Two-photon excitation fluorescence （TPEF） and second-harmonic generation （SHG） are detected through multiphoton microscopy （MPM）. The major signals have the potential to monitor the process of tissue changes. TPEF and SHG are used to monitor the skin photo-thermal response to irradiation with intense pulsed light sources （A is in the range of 560-1200 nm） and trace the process of skin remodeling in vivo at different time intervals. TPEF intensity is nearly unchanged at different time intervals after irradiation, whereas SHG intensity changes considerably. The results reveal the photo-thermal effect of nonablative light sources and the process of collagen remodeling at the sub-micron level.Two-photon excitation fluorescence （TPEF） and second-harmonic generation （SHG） are detected through multiphoton microscopy （MPM）. The major signals have the potential to monitor the process of tissue changes. TPEF and SHG are used to monitor the skin photo-thermal response to irradiation with intense pulsed light sources （A is in the range of 560-1200 nm） and trace the process of skin remodeling in vivo at different time intervals. TPEF intensity is nearly unchanged at different time intervals after irradiation, whereas SHG intensity changes considerably. The results reveal the photo-thermal effect of nonablative light sources and the process of collagen remodeling at the sub-micron level.

关 键 词：COLLAGEN IRRADIATION Light sources 

分 类 号：Q95-3[生物学—动物学]