急性等容血液稀释对兔脑缺血再灌注后早期炎症因子和S-100β蛋白的影响  

Effect of acute normovolemic hemodilution with 6% hydroxyethel starch(HES 130/0.4) on serum levels of inflammatory cytokines and S100-βprotein after cerebral ischemia reperfusion in rabbits

作　　者：郑进[1] 黄焕森[1] 庄越[1] 何雁冰[1] 

机构地区：[1]广州医学院第二附属医院麻醉科,广东广州510260

出　　处：《中国医学工程》2010年第2期10-14,共5页China Medical Engineering

基　　金：广东省科技计划项目(2006B36004011)

摘　　要：目的观察6%羟乙基淀粉(万汶130/0.4)行急性等容血液稀释对脑缺血再灌注兔血清肿瘤坏死因子-α(TNF-α)、白介素-1(IL-1)、白介素-6(IL-6)和S100-β水平的影响。方法新西兰大白兔24只随机分为3组,其中假手术组(S组,n=8);缺血-再灌注组(IR组,n=8);血液稀释-缺血-再灌注组(HIR组,n=8)。假手术组行假手术,缺血再灌注组和血液稀释缺血再灌注组经右大脑中动脉栓塞造成2小时局灶性脑缺血后再灌注;脑缺血再灌注模型参照ZeaLonga的线栓法。血液稀释缺血再灌注组从右股动脉放血,同时从股静脉补入等量6%羟乙基淀粉,20min内完成,稀释目标Hct值为30%。于缺血前30min(To)、再灌注即刻(T1)、再灌注3h(T2)、再灌注6h(T3)、再灌注24h(T4)用酶联免疫吸附法(ELISA法)检测颈静脉血清IL-1、IL-6、TNF-α、S100-β含量。结果①在T1～T4时间点,IR组和HIR组的血清TNF-α、IL-1、IL-6浓度明显高于S组(P<0.05),并且IR组比HIR组更高②与T0时比较,三组T1～T4时TNF-α、IL-1、IL-6浓度显著高于T0时(P<0.05)。③在T1～T4时间点,IR组和HIR组的血清S100-β浓度明显高于S组(P<0.01),且IR组比HIR组更高(P<0.05)。结论应用6%羟乙基淀粉血液稀释可降低脑缺血再灌注血清TNF-a、IL-1、IL-6、S100-β的表达。【Objective】To investigate the effect of acute normovolemic hemodilution with 6% hydroxyethyl starch（HES 130 /0.4） on the serum levels of tumor necrosis factor-α（TNF-α）,interleukin-1（IL-1）,inter-leukin-6（IL-6） and S100-β protein after cerebral ischemia reperfusion in rabbits.【Methods】24 New Zealand white rabbits were randomly divided into 3 groups：sham operation group（S group,n = 8）,ischemia repefusion group（IR group,n = 8）,hemodilution ischemia repefusion group（HIR group,n = 8）.Sham operation group was undergoning sham operation;ischemia reperfusion group and hemodilution ischemia reperfusion group were undergoing thread embolism of the right middle cerebral artery occlusion（MCAO） to cause focal ischemia for 2 hours and then were undergoing reperfusion;Rabbits＇cerebral ischemia reperfusion injury model was established by ZeaLonga＇s thread method.Before the ischemic period,the HIR group was undergoing acute normovolemic hemodilution（ANH） to achieve a hematocrit of 30% by stepwise blood withdraw from right femoral artery and normovolemic hemodilution with 6% HES from the femoral vein in 20 min.The jugular vein blood samples were taken for the enzyme-linked immunosorbent assay（ELISA） measurements of IL-1,IL-6,TNF-α and S100-βat before ischemia 30min（T0）,immediate reperfusion（T1）,reperfusion 3h（T2）,reperfusion 6h（T3） and reperfusion 24h（T4）.【Results】① The serum TNF-α,IL-1,IL-6 levels in the T1 ～ T4 time points were significantly higher in IR group and HIR group than those in S group（P〈0.01）.and the TNF-α,IL-1 and IL-6 serum levels were significantly higher in IR group than those in HIR group in the T1 ～ T4 time points（P 0.05） ;②Compared to T0,the the concentration of serum TNF-α,IL-1 and IL-6 were increase at T1 ～ T4 in IR group and the HIR group.（P 0.05） ;③The serum S100-β levels in the T1 ～ T4 time points were significantly higher in IR group and HIR group than those in S group（P〈0.01）,and the S1

关 键 词：羟乙基淀粉 血液稀释 缺血再灌注损伤 S100-Β蛋白 肿瘤坏死因子 

分 类 号：R392-33[医药卫生—免疫学]