体外铅染毒对人膀胱细胞基因表达谱的影响  

Differential Gene Expression of HB Cell Exposed to Lead in Culture

作　　者：孟金萍[1] 王艳蓉[1] 孙淑华[1] 杨旭[1] 刘云波[1] 

机构地区：[1]中国医学科学院实验动物研究所北京协和医学院比较医学中心卫生部人类疾病比较医学重点实验室,北京100021

出　　处：《环境与健康杂志》2010年第7期573-577,F0003,共6页Journal of Environment and Health

基　　金：国家"973"项目(2002CB51290)

摘　　要：目的研究体外培养的人胚胎膀胱组织来源细胞(humanbladder,HB)在铅暴露不同时间和浓度下的基因表达差异,以探讨铅毒性的作用机制。方法分别使用100、200μg/ml乙酸铅溶液暴露体外培养的HB细胞(浓度为2×103个/ml)8、24h,设置空白对照,提取RNA,进行cDNA基因表达谱芯片实验。芯片扫描结果经归一化处理,设定Ratio值<0.5或≥2为表达有差异基因。结果铅暴露HB细胞导致差异表达的基因共有2406个,其中,在铅暴露不同时间(8、24h)、不同浓度(100、200μg/ml)皆上调的有45个基因,皆下调的有40个基因。结论基因表达因铅暴露的时间和浓度变化而发生改变;差异表达基因的功能与信号传导、细胞代谢密切相关,也与疾病发生发展相关;可以通过分析基因表达谱改变,探悉铅暴露导致机体损伤的原因和致病机制。Objective To investigate the differential gene expression of human bladder （HB） cell exposed to lead in culture in different time and concentration,and discuss the mechanism of lead toxicity. Methods The HB cell in vitro was exposed to lead acetate at the doses of 100 and 200 μg/ml for 8 h and 24 h,and the control group was set simuhaneously. Then the total RNA of the cells was extracted for the cDNA microarrays test. The scanning data of cDNA microarray was normalized,and the differential expression genes were selected as the ratio value is t〉2 or 〈0.5. Results Two thousand and four hundred and six differential expressed genes were selected in lead-exposure HB. Fourty-five genes were up-regulated and 40 genes were downregulated in both lead concentrations （ 100,200 μg/ml） for both exposure periods（8,24 h）. Conclusion Gene expression changes with the time and lead exposure level changed. The function of differential expression genes is related to signal transduction and cellular metabolism, and also related to diseases. Through the analysis for the differential expression genes, the mechanism of leadinduced health effects can be well understood in a certain degree.

关 键 词：毒性基因组学 HB细胞 铅 CDNA芯片 聚类分析 

分 类 号：R994.6[医药卫生—毒理学]