蓓萨罗丁对热带爪蟾胚胎的致畸效应  

Teratogenic Effects of Bexarotene on Xenopus tropicalis Embryos

作　　者：张小利[1] 袁静[1] 曹庆珍[1] 刘俊奇[1] 玉琳[1] 施华宏[1] 

机构地区：[1]华东师范大学资源与环境学院上海城市化过程与生态恢复重点实验室,上海200062

出　　处：《环境与健康杂志》2010年第8期665-667,I0001,共4页Journal of Environment and Health

基　　金：国家自然科学基金资助项目(20877023);长江水环境教育部重点实验室开放基金项目(YRWEF06003)

摘　　要：目的研究蓓萨罗丁对热带爪蟾（Xenopus tropicalis）胚胎发育的影响。方法用0．5～128μg／L蓓萨罗丁对热带爪蟾胚胎进行24和48h暴露，检测热带爪蟾胚胎存活率、体长、发育阶段、畸形率和畸形指数等指标的变化。结果128μg/L蓓萨罗丁暴露48h使胚胎的存活率显著降低（P〈0．05），32或128μg/L蓓萨罗丁暴露对胚胎的生长和发育有明显的抑制作用（P〈0．05），并导致胚胎出现严重的畸形现象，主要包括胚胎脑部缩小、眼睛色素减少、围心腔水肿和尾巴弯曲等多种畸形类型。24或48h暴露后，胚胎总畸形率和眼睛畸形指数随蓓萨罗丁浓度的升高而增加（P〈0．05），32或128μg／L暴露组胚胎畸形率达100％（P〈0．001）。结论蓓萨罗丁对热带爪蟾胚胎具有极强的致畸陛。提示视黄醇类受体在热带爪蟾胚胎的发育过程中有重要作用。Objective To investigate the effects of bexarotene on fetal development of Xenopus tropicalis embryos. Methods X. tropicalis embryos were exposed to bexarotene at the doses of 0.5-128 μg/L for 24 h or 48 h, respectively. Changes of survival, body length, developmental stages and teratogenic index were observed. Results Bexarotene decreased the percent of survival of embryos at 128 μg/L after 48 h of exposure. Bexarotene significantly inhibited the growth and development of embryos at 32 or 128 μg/L. Bexarotene also led to multiple malformations, including small brains, less pigmentation in eyes, pericardial edema and bent tails. After 24 or 48 h of exposure, the total percent of malformations and eye teratogenie index were significantly increased in the treatment groups, and it reached 100% in 32 or 128 μg/L treatment groups. Conclusion Bexarotene is a strong teratogen. Our results indicate that retinoid X receptor （RXR） plays an important role in the development of X. tropicalis embryos.

关 键 词：生态毒理学 爪蟾 胚胎 致畸 

分 类 号：R994.6[医药卫生—毒理学]