尿氟的离子选择电极测定方法优化  

Optimized Determination of Urine Fluorine by Ion Selected Electrodes

作　　者：袁金华[1] 于平胜[1] 缪吉霞[1] 

机构地区：[1]南京市疾病预防控制中心理化检验科,江苏南京210003

出　　处：《环境与健康杂志》2010年第8期713-715,共3页Journal of Environment and Health

基　　金：南京市医学重点科技发展项目(ZKX06030);江苏省预防医学科研课题(Y2006026)

摘　　要：目的采用氟电极法测定尿氟，对实验过程进行全面系统的分析和优化，实现尿氟的快速准确测定。方法计算缓冲溶液中理论所需NaOH的量，并进行相关配比实验；从方法的线性范围、灵敏度、准确度、精密度等方面进行评价。结果理论计算简化了TISAB的配制过程。得出10．00ml尿样和25ml TISAB体积比条件下，模拟尿与纯水配制的样液的效果相似。大体积样品溶液提高了大电极体系的可操作性，且减少标准加入法中体积变化的影响，也减少了移液误差。标准曲线的线性范围为0．1～10．0mg／L，相关系数〉0．999，最低检测限为0．08mg／L，响应时间为0．5～5．3min，回收率为89％-114％，精密度小于10．0％。结论应用本法测定尿氟精确且干扰少，能实现复杂尿样中氟的快速准确测定。Objective To optimize the rapid method for measuring fluoride concentration in urine by ion selected fluoride electrodes. Methods The NaOH amount and mass were calculated to confect the HAc-NaAe buffer TISAB solution; the evaluations were discussed by studying the linear range, sensitivity, recovery and precision. Results The theoretical amount calculation of NaOH got the simple buffer solution preparation and the samples were advised and made of 10.00 ml urine and 25 ml TISAB, the liquid transfer error for both was decreased. The perfect lineal relation of fluoride concentrations were obtained in the range of 0.1-10.0 mg/L with the correlation coefficient above 0.999. The rates of recovery were 89%-114 %, the relative standard deviation was below 10 % and the lowest limit of detection was 0.08 mg/L. The response times varied from 0.5 to 5.3 min. Conclusion The fluoride electrodes method presents a low limit of detection, high sensitivity, accuracy and precision, without any interference and is applicable to the determination of fluoride in complicated urine samples.

关 键 词：氟化物 尿液 离子选择氟电极法 优化 

分 类 号：O657.3[理学—分析化学]