镁锌合金对成骨细胞增殖的影响  

In Vitro Effects of Mg-Zn Alloy on Proliferation in MC3T3-E1 Cells

作　　者：张岩[1] 陶海荣[2] 蒋垚[3] 张绍翔[4] 张小农[4] 李文辉[5] 

机构地区：[1]郑州大学第一附属医院骨科,郑州市450052 [2]上海交通大学附属第三人民医院骨科,上海市201900 [3]上海交通大学附属第六人民医院骨科,上海市200233 [4]上海交通大学材料科学与工程学院,上海市200240 [5]郑州大学第一附属医院,郑州市450052

出　　处：《医药论坛杂志》2010年第14期55-57,共3页Journal of Medical Forum

基　　金：国家自然科学基金资助项目(30772182)

摘　　要：目的研究镁锌合金对前成骨细胞MC3T3-E1细胞增殖的影响,探讨镁锌合金成为一种新型骨科内植物材料的可行性。方法在镁锌合金金属片上接种前成骨细胞MC3T3-E1,采用MTT法及碱性磷酸酶(ALP)检测试剂盒等方法检测MC3T3-E1细胞在材料表面的增殖活性,用左旋聚乳酸对照组。结果培养第1,3天后,镁锌合金表面成骨细胞增殖与对照组没有差异(P>0.05),第5、7、9后,镁锌合金表面成骨细胞增殖明显高于对照组(P<0.01)。结论镁锌合金能够促进MC3T3-E1细胞的增殖,可以初步认为具有良好的生物相容性。Objective To investigate the effects of Mg-Zn alloy on proliferation in MC3T3-E1 cells,and try to find out the possibility of Mg-Zn alloy to be a new type of bone surgery implant material.Methods Preosteoblast MC3T3-E1 cells were inoculated onto the Mg-Zn alloy scissel and the proliferation of MC3T3-E1 cells were measured through MTT detection.Poly-L-lactic acid （PLLA） was used as the conterparts.Results After 1 and 3 days of culture,there was no difference for the osteoblasts proliferation in two groups（P0.05）,whereas the osteoblasts proliferation on Mg-Zn alloy is significantly higher than that on the control group after 5,7 and 9 days（P0.01）.Conclusion Mg-Zn can promote the proliferation in MC3T3-E1 cells,so the Mg-Zn alloy is considered to have favourable biocompatibility.

关 键 词：镁锌合金 成骨细胞 增殖 可吸收生物材料 

分 类 号：R318.08[医药卫生—生物医学工程]