HPLC-ELSD法测定芪蛭降糖颗粒中黄芪甲苷的含量  

Content Determination of Astragaloside Ⅳ in Qizhi Jiangtang Granules by HPLC-ELSD

作　　者：李秋月[1] 张艺轩[1] 石钺[1] 

机构地区：[1]中国医学科学院药用植物研究所,北京市100193

出　　处：《中国药房》2010年第32期3056-3057,共2页China Pharmacy

基　　金：国家科技重大专项项目(2009ZX09301-003)

摘　　要：目的:建立高效液相色谱-蒸发光散射检测法(HPLC-ELSD)测定芪蛭降糖颗粒中黄芪甲苷含量的方法。方法:色谱柱为Hypersil ODS2柱(250 mm×4.6 mm,5μm),流动相为乙腈-水(32∶68),流速为1.0 mL·min-1,进样量为10μL,柱温为25℃。ELSD条件为漂移管温度110℃;气体流量为3.12 SLPM。结果:以浓度的常用对数(C)对其相应峰面积常用对数(A)进行回归分析,得回归方程为A=1.699 C+6.181,r=0.999 6(n=5);样品浓度在0.259～1.295 mg·mL-1范围内与峰面积积分值线性关系良好;最低检测限为5.18μg·mL-1;平均加样回收率为97.75%,RSD=2.17%。结论:采用PLC-ELSD法测定芪蛭降糖颗粒中黄芪甲苷含量,方法简便快捷,测定结果可靠,可用于芪蛭降糖颗粒的质量控制。OBJECTIVE：To establish HPLC-ELSD method for content determination of astragaloside Ⅳ in Qizhi jiangtang granules. METHODS：The separation was performed on Hypersil ODS2（250 mm×4.6 mm,5 μm）column with acetonitrile-water（32 ∶ 68）as mobile phase. The flow rate was 1.0 mL·min^-1. Injection volume was 10 μL. The column temperature was 25 ℃. The temperature of drift tube for measurement was 110 ℃ and flow rate of N2 was 3.12 SLPM. RESULTS：Regression equation was A=1.699 C＋6.181. The linear range of astragaloside Ⅳ was 0.259～1.295 mg·mL^-1（r=0.999 6,n=5） with lowest detectable limit of 5.18 μg·mL-1. An average recovery rate were 97.75%（RSD=2.17%）. CONCLUSION：The method is simple,rapid and reliable for quality control of Qizhi jiangtang granules.

关 键 词：高效液相色谱-蒸发光散射法 芪蛭降糖颗粒 黄芪甲苷 

分 类 号：R917[医药卫生—药物分析学]