川芎 红花 丹参及其合并提取液中亚油酸含量测定  

Determination of Linoleic Acid in Ligusticum wallichii,Carthamus tinctorius,Salvia miltiorrhiza bge and Their Amalgamative Extract

作　　者：杨春[1] 冯中宝[1] 杨金笛[2] 

机构地区：[1]贵阳中医学院药学系,贵州贵阳550002 [2]贵阳中医学院基础部,贵州贵阳550002

出　　处：《辽宁中医杂志》2010年第8期1563-1565,共3页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的:测定红花、川芎、丹参及其合并提取液中亚油酸的含量。方法:采用气相色谱法,使用HP-INNOWAX(30.0m×250μm×0.25μm)柱,Agilent6890N色谱工作站,采用外标一点法进行测定。结果:亚油酸甲酯在0.6～3.7μg内,进样量与峰面积具有良好的线性关系,r=0.9996,精密度、稳定性、重复性、回收率试验的RSD值均小于2%,平均回收率为101.57%,红花中亚油酸含量为1.004%,丹参为0.00066%,川芎为1.803%,红花/川芎为1.40%,红花/川芎/丹参为1.060%。结论:该法对于川芎等药材制剂的质量控制可提供参考。Objective：To determine the content of Linoleic Acid in Ligusticum wallichii,Carthamus tinctorius,Salvia miltiorrhiza bge and their amalgamative extract with water.Methods：Gas Chromatography,HP-INNOWAX （30.0m × 250μm × 0.25μm） column,Agilent6890N chromatographic work station and one point external standard method were used in determination.Results：The content of methyl linoleate was 0.6 ～ 3.7μg,r=0.9996,the Sample volume have a good linear relationship with the peak area,all the trial RSD of precision,stability,repeatability and recovery are less than 2%,the average recovery is 101.57%,the content of Linoleic Acid in Carthamus tinctorius is 1.004%,Salvia miltiorrhiza bge 0.00066%,Ligusticum wallichii 1.803%,the amalgamative extract of Carthamus tinctorius and Ligusticum wallichii 1.40%,the amalgamative extract of Carthamus tinctorius,Ligusticum wallichii and Salvia miltiorrhiza bge 1.060%.Conclusion：The method can be used as a reference for the quality control of Ligusticum wallichii and other medicinal preparations.

关 键 词：亚油酸 川芎 红花 丹参 气相色谱法 含量测定 

分 类 号：R284[医药卫生—中药学]