人白细胞介素-2真核表达质粒的构建及鉴定  

Construction and ldentification of the Eukaryotic Expression Plasmid Encoding Human Interleukin - 2

作　　者：陈宗德[1] 张彤[1] 张光磊[1] 张燕齐[1] 阮丽荣[1] 张绍祖[1] 

机构地区：[1]河南医科大学微生物学与免疫学教研室

出　　处：《河南肿瘤学杂志》1999年第3期177-178,共2页Henan Journal of Oncology

摘　　要：目的构建高效表达人IL－2的真核表达质粒，为IL－2的肿瘤基因治疗提供重要的工具。方法运用PCR扩增出人IL－2基因，经BamHI及EcoRI双酶切后，回收片段与BamHI及EcoRI处理的表达载体pcDNA3在T4DNA连接酶的作用下连接，并用多种限制性内切酶对重组质位进行酶切鉴定。结果PCR扩增片段的长度约为480bp，重组质粒经酶切显示，构建的质粒中含480bp的IL－2插人片段，将此重组质粒命名为pcDNA3－IL－2。结论本研究结果为进一步研究表达IL－2质粒的肿瘤基因治疗及免疫调节作用打下了基础。Objective To construct efficient expression plasmid encoding IL-2 and provide an important too, for IL-2gene therapy. Methods PCR was used to amplify a 480 bp fragment encoding full-length human IL-2.After treatment with endonucleases BamH I and EcoR I, PCR product was ligated with digested vector pcNA3 by BamH I and EcoR I. The resultant recombinant plasmid were identified with appropriate endonucleases.Results The 480 bp DNA frag ment was certified to be inserted into pcDNA3 and the recombinant plasmid was named pcDNA3-IL-2. The results have laid strong foundation for further study on the immunological effects of plasmid expressing IL-23.[

关 键 词：IL-2 表达 质粒 构建 鉴定 

分 类 号：R392.1[医药卫生—免疫学]