小鼠胸腺树突状细胞系的克隆化及基本鉴定  

Manipulation of mouse thymic dendritic cell clones and their characteristics

作　　者：裴丽红[1] 刘祝公[1] 陈慰峰[1] 

机构地区：[1]北京医科大学免疫学系

出　　处：《中国免疫学杂志》1999年第6期257-259,共3页Chinese Journal of Immunology

摘　　要：目的：应用单细胞培养系统，对本室建成的胸腺基质细胞系ＭＴＳＣ４进行细胞克隆化并鉴定。方法：在单个细胞培养中，扩增由一个细胞长成的克隆，并使之稳定生长后，进行表面标志分子和倍增时间检查，并以其分泌的细胞因子测定其生物学特性及功能。结果：获得１４个细胞克隆，它们均表达树突状细胞抗原和ＭＨＣⅡ类分子，均无角蛋白，表明各克隆均为树突状细胞，起始的ＭＴＳＣ４也是树突状细胞系。检测了细胞倍增时间和ＩＬ６、趋化因子等细胞因子，从倍增时间和因子产生能力看，各克隆差异很大。结论：从ＭＴＳＣ４细胞系克隆出性状及功能都均一的细胞克隆来看，各克隆间的细胞特性有明显区别。Objective: An attempt to develop stable mouse thymic dendritic cell clones with homogeneous characteristics was investigated by adaptation of single cell culture system. Methods: A single cell obtained from the dilution of the established mouse thymic dendritic cell line(MTSC4) was cultured in the well of Vbottomed microtiter plates until the cell cluster was grown up. The grown cell cluster was expanded as single cell clone with stable proliferation capacity.The cloned cells were characterized for their biologic properties and the profile of cytokine production. Results: A total of 14 MTSC4 clones with stable proliferation have been developed.All the cloned cells expressed MHC class II molecules and most were NLDC145+.None of the cloned cells were keratin negative.The double time and IL6 as well as chemokine production varies among different clones. Conclusion:TBZThe mouse thymic dendritic cell clones derived from respective(MTSC4) cell line have successfully been established.The biologic characteristics and cytokine production capacity were varied among different cell clones.

关 键 词：胸腺 细胞克隆 细胞因子 树突状细胞 

分 类 号：R392.12[医药卫生—免疫学]