抗子宫内膜抗体和子宫内膜异位症  

Antiendometrial Antibody and Endometriosis

作　　者：李伟毅[1,2,3] 陆勇娟[1,2,3] 朱云凤 程丽萍[1,2,3] 席晔斌[1,2,3] 陈广洁 周文达[1,2,3] 

机构地区：[1]上海第二医科大学上海市免疫学研究所 [2]上海第二医科大学附属瑞金医院妇产科 [3]上海第二医科大学医学检验实验室

出　　处：《中国免疫学杂志》1999年第6期268-270,共3页Chinese Journal of Immunology

基　　金：上海市科技发展基金

摘　　要：目的：研究抗子宫内膜抗体（ＥＭＡｂ）与子宫内膜异位症（内异症）的关系。方法：改良法提取、纯化正常人及内异症患者的子宫内膜抗原（ＥＭＡｇ），分析其氨基酸组成，并用ＳＤＳＰＡＧＥ及Ｗｅｓｔｅｒｎｂｌｏｔ分析两者蛋白质组成及抗原特异性；建立ＥＬＩＳＡ方法并测定、分析正常人及内异症患者血清ＥＭＡｂ。结果：正常人ＥＭＡｇ有２条蛋白质条带（４８０ｋＤ和大于９７．４ｋＤ）；内异症患者则有３条蛋白质条带（４６０、５４０和大于９７．４ｋＤ），前者仅１条蛋白质条带（大于９７．４ｋＤ），后者则３条蛋白质条带可与ＥＭＡｂ特异反应，且两者在氨基酸组成上亦有差异；内异症患者血清ＥＭＡｂ阳性率明显高于正常人及非内异症（其它妇科疾病）患者。结论：ＥＭＡｂ与内异症密切相关。Objective To study the relationship between the antiendometrial antibodies (EMAb) and the endometroisis. Methods: A modified isolation method of endometrial antigen (EMAg) has been developed; amino acids of the EMAg was analysed by SDSPAGE and Western Blot; the serum levels of the EMAb were detected by ELISA between the normal human and the patient with the endometriosis. Results There are two bands in the normal human EMAg, including 48 .0 kD and larger than 97.4 kD, the latter can react specifically with the EMAb. But, there are three bands in the patients with endometriosis, including 46.0 kD54.0 kD and larger than 97.4 kD, in which all three bands can react with the EMAb. In another, there are different animo acids in kind and contents in the EMAg between the normal human and patients with the endometriosis. There are significantly different in serum EMAb between the patients with the endometriosis and nonendometriosis as well as the normal human ( P <0.05). ConclusionThere are significantly relationship between the EMAb and the endometriosis.

关 键 词：子宫内膜异位症 子宫内膜抗原 抗子宫内膜抗体 

分 类 号：R711.710.3[医药卫生—妇产科学]