献血员庚型肝炎病毒感染情况和部分基因序列分析  

Detection of hepatitis G virus infection in donors and sequencing of partial genen

作　　者：赵国强[1] 邢培清[2] 苏堤[1] 胡志荣[1] 刘利民[2] 马宏伟[2] 王凯[2] 

机构地区：[1]河南医科大学微生物学与免疫学教研,郑州450052 [2]河南省红十字血液中心,郑州450003

出　　处：《河南医科大学学报》1999年第2期28-31,共4页Journal of Henan Medical University

基　　金：河南省科技攻关项目!981170404

摘　　要：为探讨河南省献血员中庚型肝炎病毒（ＨＧＶ）的感染情况和病毒基因的变异特征。方法：利 用逆转录－巢式－聚合酶链反应（ＲＴ－Ｎｅｓｔ－ＰＣＲ）检测 ＨＧＶ ＲＮＡ；克隆人 Ｔ载体并测序。结果：在 ３００名义务献血员 中发现１例庚型肝炎病毒ＲＮＡ阳性；２００例初检合格献血员标本中无１例阳性。河南株ＨＧＶ与ＧｅｎＢａｎｋ中ＨＧＶ 对应位置序列的同源性为９０.９％～９８．８％。结论：河南省义务献血员中有ＨＧＶ携带者；河南株ＨＧＶ与国内（河 北株ＨＧＵ７５３５６）分离株的同源性为９８．８％，其同源性高于国外分离株（９０．９％～９５．２％）；建议对献血员增加 ＨＧＶ 检测。Aim:In order to study hepatitis G virus(HGV)infection in voluntary donors of Henan province and characteristics of Gene mutation.Methods:HGV RNA was detected by reverse transcription nest polymerase chain reaction (RT-Nest-PCR).Partial gene was cloned and sequenced.Results:One positive case of HGV RNA was detected in 300voluntary donors and HGV rna IN 200INITIAL SCREENED DONOR WERE ALL NEGATIVE.The sequence showed 90.9%~98.8% uncleotide homology to the corresponding region of HGV in Genbank.The nucleotide homology between Henan strain HGV and Hebei is higher than that between Henan and foreign country strains.Conclusion:The existence of HGV infection in Henan has been affirmed and it is suggesting that screening for blood donors is of importance to transfusion security.

关 键 词：献血员 庚型肝炎病毒 PCR 序列分析 

分 类 号：R512.63[医药卫生—内科学]