猪肉发光细菌(PB-CS-01)的鉴定与生物学研究  

Identification and Biology Study of a Photobacterium (PB-CS-01) Causing Glowing Pork

作　　者：肖安东 邓云波 李裕 周汝顺 匡光伟 隆雪明 徐丽枚 宋跃君 陈启友 吴礼龙 

机构地区：[1]湖南省兽药饲料监察所,长沙410006

出　　处：《中国兽药杂志》2010年第8期1-4,9,共5页Chinese Journal of Veterinary Drug

摘　　要：对一株从发光猪肉中分离到的发光细菌(PB-CS-01)进行了形态结构与培养特征的观察、生化特征研究以及16S rRNA基因序列分析。结果表明,该菌为革兰氏阴性球杆菌,在温度为18～22℃及盐浓度为1.5%～3.0%的培养基中生长良好,在光照好氧及厌氧条件下都能生长,发蓝绿色光。16S rRNA基因序列分析表明该菌与GenBank中Photobacterium phosphoreum的同源性水平达100%,结合菌株的形态学和生化学特性,鉴定为Photobacterium phosphoreum。该菌对小鼠无急性致病性;对链霉素、复方新诺明和头孢呋辛等多种抗菌药物敏感;新洁尔灭、戊二醛等常用消毒剂能杀死该菌。A photobaterium strain named PB-CS-01 was isolated from commercially available glowing pork and the biological identification and characterization was also performed.Strain PB-CS-01 was characterized as a gram-negative bacterium that could be best grown in agar medium supplemented with 1.5%～3.0% salt at 18～22 ℃,and it can also grow well in aerobic conditions with light as well as in anaerobic conditions.Interestingly,this bacterium can give blue-green light.The PB-CS-01 was identified as Photobacterium phosphoreum according to its colony morphological properties,biochemical properties and the 16S rRNA sequence analysis.Pathogenic assay and drug-sensitivity test indicated that PB-CS-01 has no acute pathogenicity to mouse and highly sensitive to antibiotics,such as streptomycin,cotrimoxazole,cefuroxime,etc.

关 键 词：发光细菌 细菌鉴定 16S RRNA基因序列分析 药敏实验 急性致病性实验 

分 类 号：Q93-331[生物学—微生物学]