新型荧光光源吖啶橙染色和QBC技术快速诊断间日疟的现场评价  

Field Evalluation of QBC Technique and AO Method Applied to Rapid Diagnosis of Vivax Malaria

作　　者：刘佩娜[1,2,3,4] 王兴振[1,2,3,4] 朱声华 田玉[1,2,3,4] 廖琳 刘庆[1,2,3,4] 川本文彦 王述琼[1,2,3,4] 何远东 

机构地区：[1]华西医科大学寄生虫学教研室 [2]日本名古屋大学国际保健医疗学教研室 [3]四川省名山县卫生防疫站 [4]四川省筠连县卫生防疫站

出　　处：《地方病通报》1999年第2期41-43,共3页Endemic Diseases Bulletin

基　　金：联合国开发计划暑/世界银行/世界卫生组织热带病研究与培训特别规划(TDR)资助

摘　　要：：１９９２～１９９８年期间用新型荧光光源，薄血膜吖啶橙染色法（ＡＯ法）和吖啶橙包被的毛细管法（ＱＢＣ技术），分别在四川省筠连县和名山县疟疾流行区，对４５２８人进行了现场应用，其中１４５例采用ＡＯ法和ＱＢＣ技术同时检测，并用姬姆萨染色作对照。ＡＯ法与ＱＢＣ技术的特异性、敏感性和符合率分别是９７．２％、９１．２％、９４．３％和９５．３％、９４．８％、９５．０９％，三种方法每个标本平均检测时间分别为０３０″、１０７″和１０００″The present study was done in Sichuan, China, a main endemic area of vivax malaria with low parasitemia, during the transmission seasons form 1992 to 1998. 300ul blood was connected from each subject to fill QBC tube and to prepare blood smears. The Giemsa thick smear was made with 10ul blood spreading evenly over a 12mm area. Each QBC tube was observed for 5min, and each blood smear was examined for 300 microscopic fields. If no parasite was found, the sample was taken as confident negative. The minimum time required for each positive diagnosis was measured precisely with a stopwatch.\;4 528 volunteer villagers and suspected malaria patients in the local clinics, aged 4 months to 88 years, were examined. Which 145 volunteer taking the Giemsa thick smear as a standard, the sensitivity, specificity and concordance of the QBC and AO stained thin smear were 95.3%, 94.8%,95.0%,and 97.2%, 91.2%, 94.3%, respectively. The median of the minimum time required for positive results by the Giemsa thick smear, QBC and AO method were 1000, 107and 030 respectively. Which 3657 volunteers taking AO method from 1996 to 1998 result 66 positive. Comparing which QBC technique, AO method also possesses such obvious advantages as easy manipulation, low cost per case and much more ideal in speed for rapid diagnosis of vivax malaria under field conditions. \ \

关 键 词：间日疟 吖啶橙色法 疟疾 诊断 

分 类 号：R531.304[医药卫生—内科学]