茶卷叶蛾寄生真菌的分离、鉴定及毒力测定  

Isolation,identification and virulence of Beauveria bassiana from a dead Homona coffearia

作　　者：王定锋[1] 吴光远[1] 王庆森[1] 刘丰静[1] 曾明森[1] 

机构地区：[1]福建省农业科学院茶叶研究所,福建福安355015

出　　处：《福建农业学报》2010年第3期345-349,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省茶产业技术体系建设专项;福建省科技计划重点项目(2007N0027);福建省农业科学院青年人才基金项目(2009QB-14);福建省农业科学院科技下乡"双百"行动计划项目(sbmx0910)

摘　　要：从茶园中自然羅病而死的茶卷叶蛾(Homona coffearia)幼虫僵虫上分离纯化出1株病原真菌,经核糖体DNA(rDNA)转录间隔区(internal transcribed spacer,ITS)鉴定为球孢白僵菌(Beauveria bassiana),并命名为JYBb201菌株。对该菌株在PDA、PPDA和SDAY3种培养基上的生长速度和产孢能力进行测定,发现其在PDA上平均生长速度最快,菌丝的日均增长量达4.30 mm。但在SDAY培养基上培养16 d的产孢量比其他两种培养基大,达4.30×107孢子.mL-1。对茶卷叶蛾幼虫的毒力测定结果显示,该菌具有较高的杀虫活性,以2.16×108孢子.mL-1和4.32×107孢子.mL-1的菌液处理,9 d后其校正死亡率均达到94.74%,表明JYBb201菌株是1株对茶卷叶蛾幼虫具有潜在防治作用的病原真菌菌株。Through a series of cultivations and purifications,a pathogenic fungus was isolated from the dead body of a Homona coffearia Meyrick in a tea plantation.It was subsequently identified to be Beauveria bassiana by means of rDNA-ITS sequencing,and named JYBb201.The colony diameter and sporulation of JYBb201 on three media（i.e.,PDA,PPDA and SDAY） were observed.The results showed that the JYBb201 colonies grew fastest on PDA among the three media with a daily increase of 4.3 mm in diameter.However,the highest sporulation occurred on SDAY after cultivation for 16 days.The virulence of JYBb201 was tested by treating H.coffearia larvae with a suspension of fungal spores at five concentration levels.When the larvae were dealed with a suspension of fungal spores with 2.16×108 mL-1 and 4.32×107 mL-1 respectively,the two corrected mortalities of larvae were both 94.74% after 9 days.The results indicated that JYBb201 could be a potential pathogenic microorganism for controlling the pest.

关 键 词：茶卷叶蛾 球孢白僵菌 分离鉴定 RDNA-ITS 生物学 毒力 

分 类 号：S435[农业科学—农业昆虫与害虫防治]