GFP基因转化藿香的研究  

Transfering Green Fluorescent Protein Gene into Agastache rugosa (Fisch.et Mey).Kuntze

作　　者：程志号[1] 惠荣奎[1] 蔡明历[1] 郝大翠[1] 刘焰[1] 

机构地区：[1]华中农业大学药用植物研究所,武汉430070

出　　处：《生物技术通报》2010年第8期116-119,共4页Biotechnology Bulletin

基　　金：华中农业大学创新基金项目

摘　　要：以藿香无菌苗幼嫩茎段为受体,利用根癌农杆菌介导法进行了绿色荧光蛋白基因(GFP)的遗传转化研究。经农杆菌侵染,通过共培养、选择培养后获得其抗性愈伤组织,对抗性愈伤组织的诱导过程进行了GFP荧光检测。结果表明,GFP基因能在抗性愈伤组织中强烈表达,证明GFP基因能够在藿香遗传转化中得到应用。对抗性愈伤组织的PCR检测初步证实外源GFP基因已整合到藿香愈伤组织的基因组中。In order to confirm its practical value of the green fluorescent protein（GFP）,a visual marker,in Agastache rugosa（Fisch.et Mey）.Kuntze transformation,Agrobacterium tumefaciens-mediated transformation by using young stem as explants was investigated.GFP detection was conducted during the transformation and strong GFP expression was observed in putative transformation which indicated that GFP could be used in Agastache rugosa（Fisch.et Mey）.Kuntze transformation.Results of PCR proved preliminarily that the GFP gene had integrated into Agastache rugosa（Fisch.et Mey）.Kuntze calluses genome.

关 键 词：藿香 GFP基因 根癌农杆菌 PCR 

分 类 号：S573.9[农业科学—作物学]