蒙古冰草MwLEA3基因植物表达载体的构建  

Construction of Plant Expression Vector of the Mongolian Wheatgrass MwLEA3 Gene

作　　者：石凤敏[1] 云锦凤[1] 赵彦[1,2] 逯晓萍[1] 

机构地区：[1]内蒙古农业大学生态环境学院,呼和浩特010018 [2]内蒙古农牧业科学院,呼和浩特010031

出　　处：《生物技术通报》2010年第8期125-128,共4页Biotechnology Bulletin

基　　金："973"项目子课题(2007CB108901-1);国家自然科学基金资助项目(30760159);内蒙古草业重大专项项目(20071923-1-1)

摘　　要：以植物双元表达载体pCAMBIA2300为基础,设计分别带有酶切位点XbaI和PstI的一对引物,从克隆载体pGM-T-MwLEA3中扩增到目的基因MwLEA3。用XbaI和PstI双酶切该目的基因及表达载体pCAMBIA2300,回收后利用T4DNA连接酶连接,获得植物表达载体pCAM-MwLEA3。通过冻融法将所获得的植物表达载体重组质粒导入根癌农杆菌LBA4404菌株中,为该基因的功能鉴定及通过农杆菌介导法将MwLEA3基因导入植物提高相关抗性奠定了基础。Mongolian wheatgrass Late Embryogenesis Abundant gene（MwLEA3） was amplified using specific primers containing restriction enzyme site of Xba I and Pst I.PCR products and the binary expression vector pCAMBIA2300 were digested by the corresponding restricted enzymes respectively,and linked by T4 DNA ligase.The resulting construction with MwLEA3 was named pCAM-MwLEA3.The pCAM-MwLEA3 was transferred into Agrobacterium tumefaciens strain LBA4404 with freeze-thaw method for the following-up research,such as gene functional identification and gene transformation for other plants stress tolerance improvement.

关 键 词：蒙古冰草 MwLEA3基因 植物表达载体 

分 类 号：S543.9[农业科学—作物学]