转P_(rd29A)-ipt基因对烟草根及侧芽生长的影响  

Effects of Transforming P_(rd29A)-ipt Gene on Growing of Tobacco Lateral Bud and Root

作　　者：董绍旺[1] 王爱英[1] 孙建富[1] 沈海涛[1] 祝建波[1] 

机构地区：[1]石河子大学农业生物技术重点实验室,石河子832000

出　　处：《生物技术通报》2010年第8期137-140,共4页Biotechnology Bulletin

基　　金：国家转基因专项(2008ZX08011-002;2008ZX08005-004)

摘　　要：利用异戊烯基转移酶(isopentenyl-transferases,ipt)基因转化植物,可明显提高抗逆性和抗病性,延缓叶片衰老。逆境胁迫常会对植物造成很大的伤害,拟南芥的Prd29A启动子受干旱、盐及冷胁迫诱导。构建了Prd29A-ipt植物表达载体,通过农杆菌介导转化普通烟草,获得了能够正常生长的转基因植株。通过表型观察发现,转Prd29A-ipt烟草具有一定的组成型表达,降低了烟草的顶端优势,促进了烟草侧芽的生长,同时,对根的伸长有一定的抑制作用。Transgenic plants with isopentenyl transferase gene（ipt） can improve the ability of stress-and disease-resistance,and delay the senescence of leaves.Stress usually causes great damage to plants.Prd29A of Arabidopsis thaliana is drought-salt-and cold-inducible promoter.We constructed Prd29A-ipt plant expression vector,transformed tobacco by Agrobacterium tumefaciens,and have acquired a few transgenic tobaccos.By detecting phenotype,we found that tobaccos transformed Prd29A-ipt could constructively express,decrease apical dominance and improve lateral bud growing,but inhabit root elongation.The study might provide referece for further study in graft plants such as flowers and fruit trees.

关 键 词：异戊烯基转移酶 细胞分裂素 顶端优势 侧芽 

分 类 号：S572[农业科学—烟草工业]