槟榔黄化病组织RNA提取方法的比较  

Comparison of Methods for Extracting Total RNA from Yellow Leaf Disease Tissue of Areca catechu L.

作　　者：周亚奎[1,2] 杨云[1,2] 隋春[3] 甘炳春[1,2] 魏建和[3] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所海南分所,万宁571533 [2]海南省南药资源保护与开发重点实验室,万宁571533 [3]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《生物技术通报》2010年第8期153-156,共4页Biotechnology Bulletin

基　　金："十一五"国家科技支撑计划项目(2007BAI27B03)

摘　　要：为了从发生黄化病的槟榔茎、叶、花中提取到完整的RNA,采用了CTAB法、Trizol法和异硫氰酸胍法提取槟榔黄化病组织RNA。从RNA的完整性、产率和纯度方面对这几种方法进行了比较,结果表明,异硫氰酸胍法所得RNA完整性较好,条带清晰无明显降解,OD260/OD280介于1.8-2.0之间,RNA得率较高,为120μg/g以上,并能成功进行反转录制备cDNA,表明该RNA可以进行后续分子生物学操作。In order to extract pure and integrate RNA from stem,leaf and flower of Areca catechu L.,the CTAB,Trizol and guanidine isothiocyanate method were compared.According to the integrity,productivity and purity of extracted RNA,the guanidine isothiocyanate method was the most appropriate method of RNA extraction for Areca catechu L.With this method,the RNA bands were clear without degradation,the ratio of OD260/OD280were 1.8-2.0,and yield of total RNA 120 μg/g.The RNA was suitable for RT-PCR and cDNA synthesis,and can be used for the following-up molecular biology operations.

关 键 词：槟榔 黄化病 RNA提取 异硫氰酸胍法 

分 类 号：S763.7[农业科学—森林保护学]