丝胶蛋白/羟基磷灰石复合支架材料的制备及性能研究  

Preparation and Performance of Silk Sericin/Hydroxyapatite Composite Bone Scaffold

作　　者：王琳婷[1] 杨明英[1] 朱良均[1] 张海萍[1] 闵思佳[1] 邓连霞[1] 

机构地区：[1]浙江大学应用生物资源研究所,杭州310029

出　　处：《蚕业科学》2010年第4期639-644,共6页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金项目(No.30771633)

摘　　要：羟基磷灰石(HA)是骨组织中无机物的主要成分。利用丝胶蛋白(SS)良好的生物相容性、生物降解性、细胞相容性,以及含有的羧基和羟基能与Ca2+紧密结合的特点,将氢氧化钙和磷酸以湿法合成的羟基磷灰石按一定的比例加入到浓缩后的丝胶蛋白溶液中,经冷冻干燥制备成丝胶蛋白/羟基磷灰石复合支架材料,期望用于骨替代和骨缺损修复。对丝胶蛋白/羟基磷灰石复合支架材料进行扫描电镜(SEM)、X射线衍射(XRD)、红外吸收光谱(FTIR)、热力学性能以及力学性能等检测,并探讨不同原料配比对材料结构与性能的影响。结果表明,丝胶蛋白/羟基磷灰石复合支架材料的孔隙分散均匀,孔隙率33.0%～62.5%;支架材料中的HA呈弱结晶态,与人体骨组织中HA的晶体态相似,丝胶蛋白分子呈β折叠结构;随着复合支架材料中HA的比例不断增加,材料的热分解温度提高,热学性能改善,当HA的质量分数达到50%时,弹性模量增大到15.64MPa,呈现较好的结构性能。Hydroxyapatite （HA） is the main component of inorganic matters in bone tissue.Silk sericin （SS） has good biocompatibility,biodegradability and cellular compatibility with bone tissue.It also has the hydroxyl and carboxyl groups that can have close association with Ca^2＋ .In order to utilize these characteristics for bone repairing and bone replacement,we prepared HA through co-precipitation of Ca（OH）2 and H3PO4 and added it into concentrated SS solution in proportion.By means of freeze-drying,the SS/HA composite scaffold material was made.The SS/HA scaffold material was characterized by scanning electronic microscopy （SEM）,fourier transform infrared spectrometer （FTIR）,X-ray diffractometer （XRD）,thermodynamic performances and mechanical properties etc.And influence of different raw material ratio to the structure and performance of SS/HA scaffold was investigated.The results revealed that pores in the scaffolds were well-distributed.The porosity rate was 33.0%～62.5%.HA in the scaffolds were of slight crystallization,similar to crystal morphology of HA in human bone.Sericin molecules were in β-sheet structure.With the increase of HA pro-portion in the composite scaffold material,the material＇s decomposition temperature raised,and the thermodynamic performance was improved.When the mass fraction of HA reached 50%,the material＇s modulus of elasticity was increased to 15.64 MPa,showing favorable structural properties.

关 键 词：骨组织修复材料 丝胶蛋白 羟基磷灰石 复合支架材料 原料配比 结构性能 

分 类 号：R318.08[医药卫生—生物医学工程]