从云南省地方黄茧品种B黄2克隆的类胡萝卜素结合蛋白基因的cDNA序列分析  

cDNA Sequence Analysis of Carotenoid-binding Protein Coding Gene Cloned from B huang 2,a Local Yellow Cocoon Variety of Yunnan Province

作　　者：白兴荣[1] 付坚[2] 廖鹏飞[1] 董占鹏[1] 黄平[1] 

机构地区：[1]云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101 [2]云南省农业科学院生物技术与种质资源研究所,昆明650223

出　　处：《蚕业科学》2010年第4期687-691,共5页ACTA SERICOLOGICA SINICA

基　　金：云南省自然科学基金项目(No.2007C234M)

摘　　要：类胡萝卜素结合蛋白(CBP)是家蚕黄茧形成的关键因子。利用RT-PCR方法从云南省地方黄茧品种B黄2的5龄幼虫丝腺中克隆了编码CBP的基因cDNA序列。序列及同源性分析表明,克隆的CBP基因cDNA序列全长898bp,编码297个氨基酸残基组成的蛋白,该序列与从日本黄茧品种N4的丝腺中克隆的编码CBP的基因(GenBank登录号:AB062740)序列相似性为100%。生物信息学分析表明该序列编码的CBP蛋白为非分泌型蛋白,且定位于细胞质中的可能性最大。研究结果显示云南黄茧品种B黄2与日本黄茧品种N4的CBP基因CDS序列完全一致,编码蛋白也一样,证明黄茧品种B黄2的茧色不同于N4的茧色,并非是由CBP基因序列产生变异位点导致的,色彩的差异可能是受到黄茧系其它茧色形成因素的影响。Carotenoid-binding protein （CBP） is the key factor leading to the formation of Bombyx mori yellow cocoon.In present study,RT-PCR method was employed to clone the cDNA sequence encoding Bombyx mori CBP from silk gland of the 5th instar larvae of B huang 2,a local yellow cocoon variety of Yunnan Province.Sequence analysis showed that the full-length cDNA of Yunnan Bombyx mori CBP is 898 bp long,encoding 297 amino acid residues.Homology analysis showed that sequence identity of the cloned cDNA is 100% with the CBP gene cloned from silk gland of N4,a Japanese yellow cocoon variety （GenBank accession No.AB062740）.Bioinformatics analysis indicated that Yunnan Bombyx mori CBP is not a secretory protein,and is most likely located in the cytoplasm.The above results showed that the CDS and protein sequences of Yunnan yellow cocoon variety in Yunnan and those in the Japanese N4 variety are exactly the same,confirming that color variation of Yunnan yellow cocoon variety with Japanese N4 is not caused by mutation in the CBP protein.The color difference is probably caused by mutations in other genes of yellow cocoon variety.

关 键 词：家蚕黄色茧 云南地方品种 类胡萝卜素结合蛋白 基因克隆 序列分析 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q78[农业科学—畜牧兽医]