PELGE空白纳米粒的体外免疫学评价  

In vitro immunotoxicology investigation on blank PELGE nanoparticles

作　　者：何黎黎[1] 杨立开[2] 邓黎[2] 张志荣[2] 龚涛[2] 孙逊[2] 

机构地区：[1]西南民族大学化学与环境保护工程学院,四川成都610041 [2]四川大学华西药学院靶向药物与释药系统教育部重点实验室,四川成都610041

出　　处：《华西药学杂志》2010年第4期400-402,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的评价mPEG-PLGA-mPEG(PELGE)空白纳米粒的体外免疫性。方法采用定量酶联检测与PELGE纳米粒共同培养后的THP-1源巨噬细胞细胞因子IL-6、IL-1β和TNF-α的释放量。结果 9种PELGE材料所制备的纳米粒中,9号材料(PEG2000、5%、LA/GA=5:5)可以导致THP-1源巨噬细胞IL-6分泌量显著高于阴性对照组和PLGA纳米粒组。结论 9号材料可能有潜在的免疫毒性,而其余8种材料具有较好的免疫相容性。OBJECTIVE To investigate the immunotoxicity of blank mPEG-PLGA-mPEG（PELGE） nanoparticles.METHODS ABC-ELISA methods were applied to measure the release of IL-6,IL-1β and TNF-α by THP-1 cells after the incubation with blank PELGE nanoparticles.RESULTS Among the tested 9 kinds of PELGE nanoparticles,only the No.9 nanoparticles（PEG 2000,5%,LA/GA = 5：5） could cause significant increase in the release of IL-6 factor by THP-1 cells compared with that of blank control and PLGA nanoparticles group.CONCLUSION No.9 PELGE polymer might have potential immunotoxicit,while the other 8 ones might not.

关 键 词：PELGE 纳米粒 体外免疫学 

分 类 号：R94[医药卫生—药剂学]