核酸探针斑点杂交检测对虾传染性肌肉坏死病毒  

Detection of Infectious Myonecrosis Virus by Dig-DNA Probe Dot-Blot Hybridization

作　　者：谢玮[1] 闫冬春[1] 牛余泽[1] 孟莳[1] 赵军[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025

出　　处：《水产科学》2010年第8期443-446,共4页Fisheries Science

基　　金：山东省高等学校优秀骨干教师国际合作培养项目(2007);山东省高等学校科技计划项目(J09LC01)

摘　　要：通过PCR方法制备了对虾传染性肌肉坏死病毒地高辛标记探针,探针长度123 bp,通过核酸探针斑点杂交检测方法对此探针特异性及灵敏度进行验证。结果表明,该方法检测对虾传染性肌肉坏死病毒cDNA的检出灵敏度为108拷贝,可检出1拷贝的传染性肌肉坏死病毒cDNA的PCR产物。本方法可应用于健康亲虾、苗种的培育和无特定病原(SPF)对虾种群的选育及传染性肌肉坏死病毒流行病学调查,具有较高的应用价值。IMNV（Infectious myonecrosis virus,IMNV）probe labeled by digoxigenin was synthesized by PCR.The DIG-labeled fragment was 123 bp in size,and the sensitivity and specificity of this probe were tested using dot blot hybridization.The results showed that the sensitivity of this probe to IMNV cDNA was 108 copies and tested 1 copy of IMNV cDNA PCR product.This research was found a highly-applied value in detecting IMNV,diagnosing epidemic disease and specific pathogen free（SPF）shrimp breeding.

关 键 词：对虾传染性肌肉坏死病毒 PCR 斑点杂交 探针 

分 类 号：S945.49[农业科学—水产养殖]