杭州湾与海州湾彩虹明樱蛤遗传差异的ISSR分析  

Genetic Variation in Geographical Populations of Clam Moerella iridescens from Hangzhou Bay and Haizhou Bay Based on ISSR Technique

作　　者：董志国[1,2] 李晓英[1] 王美珍 陈汉春 孟学平[1] 程汉良[1] 阎斌伦[1] 李家乐[2] 

机构地区：[1]淮海工学院江苏省海洋生物技术重点实验室,江苏连云港222005 [2]上海海洋大学水产与生命学院,上海201306 [3]慈溪市水产研究所,浙江慈溪315300

出　　处：《水产科学》2010年第8期460-464,共5页Fisheries Science

基　　金：国家"863"计划项目(2006AA10A410);宁波市农业与社会发展科研攻关项目(2005C100035);淮海工学院校自然科学基金资助项目(Z2005018)

摘　　要：利用ISSR分子标记技术对慈溪、乍浦、连云港3个彩虹明樱蛤地理群体的遗传差异进行了研究。试验结果表明,乍浦、慈溪、连云港3个彩虹明樱蛤群体的遗传多样性指数分别为0.3987、0.3670、0.4084,以连云港群体遗传多样性较高,慈溪群体最低。群体遗传结构显示,大多数(94.75%)的遗传多样性是由群体内不同个体间的差异造成的,只有小部分(5.25%)的遗传多样性与群体间的遗传分化有关。Nei’s遗传多样性分析结果表明,群体结构水平较低而基因流水平较高(Gst=0.0525;Nm=9.0295),提示彩虹明樱蛤群体间可能发生过较强的基因交流。The genetic diversity of clam Moerella iridescens collected from Lianyungang（Haizhou bay）,Cixi and Zhapu（Hangzhou bay）was studied using ISSR（Inter Simple Sequence Repeats）molecular technology.In Shannon index,there was the genetic diversity of 0.3987 for the Zapu population,0.3670 for the Cixi population and 0.4084 for the Lianyungang population.Population structure analysis showed that the most genetic variation was derived from the intra-populations（94.75%）,and very few from the inter-populations（5.25%）.Analysis of Nei＇s genetic diversity showed that there was a low level of population genetic structure with a considerable frequency of gene flow（Gst =0.0525;Nm = 9.0295）.This result cues that a strong gene exchange might have occurred among the populations.

关 键 词：彩虹明樱蛤 ISSRS 遗传差异 遗传结构 

分 类 号：S968.317[农业科学—水产养殖]