小鼠尾静脉注射与断头取血实验技术的改进  

Improved Experimental Techniques of Caudal Vein Injection and Getting Blood from Decapitation in Mouse

作　　者：苏丽娜[1] 郭剑伟[1,2] 饶光玲[2] 

机构地区：[1]大理学院药学院,云南大理671000 [2]贵阳医学院药学院,贵阳550004

出　　处：《大理学院学报（综合版）》2010年第6期25-27,共3页Journal of Dali University

基　　金：国家自然科学基金资助项目(30801439)

摘　　要：目的：改进小鼠尾静脉注射与断头取血实验技术。方法：采用自制固定器固定ICR小鼠,热水浴扩张尾静脉后注射给药,随后断头取血收集血样。结果：每只注射完成时间约2min,1min完成断头取血,可收集到血样0.7～1.1mL。结论：通过上述实验技巧可明显提高小鼠尾静脉注射成功率和效率,快速收集到较多的血样。Objective：Techonlogical improvements of caudal vein injection and getting blood from decapitation in mouse.Method： ICR mouse was fixed by a fixator made by ourselves and the caudal vein was expanded by warm water bath,and then the drug was administrated by caudal vein injection,blood was collected from decapitation.Result：The mean time of completed caudal vein injection and getting blood from decapitation was 2 min and 1 min respectively,0.7-1.1mL blood could be collected.Conclusion： Achievement ratio and efficiency of caudal vein injection in mouse could be improved obviously through this experimental skill,and more blood sample could be collected.

关 键 词：小鼠 尾静脉注射 断头取血 

分 类 号：Q959.837[生物学—动物学]