尖峰岭抗逆性根瘤菌的筛选及其16S rDNA序列的测定  

A Preliminary Study on Screening Adversity Resistance of Rhizobium Strains in Jianfengling Reserve and the 16S rDNA Determination

作　　者：彭玉红[1] 焦如珍[1] 牟新涛[2] 

机构地区：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091 [2]中国林业科学研究院森林生态环境与保护研究所,北京100091

出　　处：《林业科学研究》2010年第4期530-536,共7页Forest Research

基　　金：国家科技基础条件平台项目“林业微生物菌种资源标准化整理、整合及共享试点”子项目(编号2005DKA21207)

摘　　要：为了探讨木本豆科植物根瘤菌对不良环境的抵抗能力,对分离自海南尖峰岭国家自然保护区的9株根瘤菌进行了初步研究。结果发现：9株根瘤菌最适生长pH值均为7,最适NaCl含量均为10.0 g.kg-1;菌株CAF224的最适生长温度为37℃,菌株CAF416、CAF438和CAF279的最适温度为20-28℃,其余5株为28℃。9株根瘤菌均可在pH值4-11、NaCl 20.0 g.kg-1、37℃条件下生长,其中CAF224和CAF276菌株耐受pH值3;CAF226可以在40.0 g.kg-1NaCl培养基中生长;CAF276可耐受20 min 50℃、10 min 60℃高温。不同宿主、不同生态条件下的根瘤菌株具有不同的抗逆性,通过综合分析,筛选出4株高抗菌株（CAF226、CAF276、CAF224、CAF414）。16S rDNA序列系统发育分析表明：9菌株在系统进化树上与GenBank中序列号为EF054889的Rhizobium tropiciClone H12（热带根瘤菌）聚为一族,相似度为99.4%,初步确定9株根瘤菌为热带根瘤菌。In order to identify the resistance abilities of woody Legume Rhizobium,9 strains isolated from roots nodules of different legume plants in the Jianfengling National Nature Reserve,Hainan Province,were preliminary studied.Results show that the optimal pH and NaCl concentration for growth are respectively 7 and 10.0 g·kg-1 for all the 9 isolates,but the temperatures are varied,the optimal growth temperature for CAF224 is 37 ℃,for CAF416,CAF438 and CAF279 is between 20-28 ℃,and for the other 5 strains is 28 ℃.All the 9 isolates can grow under pH 4-11,20.0 g·kg-1 NaCl,and at 37 ℃,of which the isolates CAF224 and CAF276 could tolerate pH 3,while CAF226 could grow at 40.0 g·kg-1 NaCl;CAF276 could survive the high temperature of 50 ℃ for 20 min.and 60 ℃ for 10 min.The Rhizobium strains from different hosts and under different ecological conditions demonstrate different adversity resistances.4 strains have higher resistances,which are CAF226,CAF276,CAF224 and CAF414.A phylogenetic analysis based on 16S rDNA gene sequences reveals that the 9 strains gather a cluster with Rhizobium tropici Clone H12（GenBank EF054889） with a similarity of 99.4%.The 9 strains presented here are tentatively identified as Rhizobium tropici Martinez-Romero et al.

关 键 词：尖峰岭 耐盐性试验 pH范围 抗逆性 16S RDNA序列 热带根瘤菌 

分 类 号：S711[农业科学—林学]