农杆菌介导的SeNHX1基因转化月季愈伤组织的研究  

Studies on Agrobacterium-mediated Transformation of Rose Callus with SeNHX1 Gene

作　　者：刘会超[1] 刘孟刚[1] 郭丽娟[2] 贾文庆[1] 

机构地区：[1]河南科技学院园林学院,河南新乡453003 [2]河南科技学院新科学院,河南新乡453003

出　　处：《林业科学研究》2010年第4期617-621,共5页Forest Research

基　　金：河南省基础与前沿研究项目(082300430030);创新人才工程项目(2005126-49);河南科技学院博士基金资助项目(HK05-1226)

摘　　要：’Pink Peace’rose was used to study the optimum conditions for transferring the SeNHX1 gene into the callus.The results showed that the optimal medium was MS+2,4-D 5.0 mg·L-1 + TDZ 0.5 mg·L-1.Agrobacterium tumefaciens-mediated transformation was able to take the target gene into callus and the blue spots were found.The optimum conditions for the transient expression of gusA gene are as following: bacterium density of OD was 0.5,infection time was 20 min,culture time was 3 days.Adding 100 μmol·L-1 AS,the frequency of transient expression of GUS gene was the highest,which reached about 85% in present study.＇Pink Peace＇rose was used to study the optimum conditions for transferring the SeNHX1 gene into the callus.The results showed that the optimal medium was MS＋2,4-D 5.0 mg·L-1 ＋ TDZ 0.5 mg·L-1.Agrobacterium tumefaciens-mediated transformation was able to take the target gene into callus and the blue spots were found.The optimum conditions for the transient expression of gusA gene are as following： bacterium density of OD was 0.5,infection time was 20 min,culture time was 3 days.Adding 100 μmol·L-1 AS,the frequency of transient expression of GUS gene was the highest,which reached about 85% in present study.

关 键 词：月季 遗传转化 农杆菌介导 SeNHX1基因 

分 类 号：S722.3[农业科学—林木遗传育种]