郁金香授粉后雌蕊生理生化变化的初步研究  

Study on Physiological and Biochemical Changes of Tulip Pistil after Pollination

作　　者：王彩霞[1] 欧阳彤[1] 姜彦成[2] 栾启福[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400 [2]新疆大学生命科学与技术学院,新疆乌鲁木齐830046

出　　处：《林业科学研究》2010年第4期622-625,共4页Forest Research

基　　金：国家自然科学基金项目(30560016)

摘　　要：The protective enzyme(SOD,POD,CAT) activities and soluble protein content of pistil after pollination were compared between interspecific-cross and self-cross of cultivar,using Tulipa specie(T.tianschanica) from Xinjiang and T.gesneriana cultivars(’Kees Nelis’,’Negrita’) as test materials.The results showed that all the SOD,POD,CAT activities and soluble protein content changed after interspecific and self pollination,which means that the recognition between pollen and stigma can be initiated effectively when pollen fell on stigma despite of genetic relationship.Fluctuant changes were observed in 5 hours after pollination for protective enzyme activities and soluble protein content,and the fluctuation tendencies were different between interspecific and self pollination,as well as between ’Kees Nelis’ ×T.tianschanica and ’Negrita’×T.tianschanica.Self pollination appeared peaks of POD and CAT activities preceding interspecific pollination.Self pollination showed a larger rise and maintained rise for longer time than interspecific pollination.The SOD activity of ’Kees Nelis’ ×T.tianschanica changed more than that of ’Negrita’×T.tianschanica,meanwhile,the soluble protein content of the former declined preceding that of the latter.These differences were probably attributed to genetic relationship.The protective enzyme（SOD,POD,CAT） activities and soluble protein content of pistil after pollination were compared between interspecific-cross and self-cross of cultivar,using Tulipa specie（T.tianschanica） from Xinjiang and T.gesneriana cultivars（＇Kees Nelis＇,＇Negrita＇） as test materials.The results showed that all the SOD,POD,CAT activities and soluble protein content changed after interspecific and self pollination,which means that the recognition between pollen and stigma can be initiated effectively when pollen fell on stigma despite of genetic relationship.Fluctuant changes were observed in 5 hours after pollination for protective enzyme activities and soluble protein content,and the fluctuation tendencies were different between interspecific and self pollination,as well as between ＇Kees Nelis＇ ×T.tianschanica and ＇Negrita＇×T.tianschanica.Self pollination appeared peaks of POD and CAT activities preceding interspecific pollination.Self pollination showed a larger rise and maintained rise for longer time than interspecific pollination.The SOD activity of ＇Kees Nelis＇ ×T.tianschanica changed more than that of ＇Negrita＇×T.tianschanica,meanwhile,the soluble protein content of the former declined preceding that of the latter.These differences were probably attributed to genetic relationship.

关 键 词：郁金香 种间杂交 保护酶 蛋白质 

分 类 号：S682.263[农业科学—观赏园艺]