低剂量辐射诱导小鼠胸腺细胞及其亚组的适应性反应  

Adaptive response of thymocytes and its subsets induced by low dose whole body irradiation with X rays in mice\

作　　者：苏旭[1] 刘建香[1] 张迎春[1] 鞠桂芝[1] 刘树铮[1] 

机构地区：[1]白求恩医科大学卫生部放射生物学重点实验室

出　　处：《中华放射医学与防护杂志》1999年第3期174-176,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金

摘　　要：目的研究低剂量辐射预先照射以及随后大剂量照射后小鼠胸腺细胞Ｔ细胞受体（ＴＣＲ）、ＣＤ３、ＣＤ４和ＣＤ８分子表达的变化。方法ＴＣＲ、ＣＤ３表达采用间接荧光流式细胞术检测，ＣＤ４、ＣＤ８表达采用双参数直接免疫荧光流式细胞术检测。结果实验结果表明：单纯１５ＧｙＸ射线全身照射后ＴＣＲ，ＣＤ３阳性细胞数以及ＣＤ４－Ｄ８－，ＣＤ４＋ＣＤ８＋，ＣＤ４＋ＣＤ８－和ＣＤ４－ＣＤ８＋细胞数明显减少，当１５ＧｙＸ射线全身照射前６小时预先照射００７５Ｇｙ时，可明显减轻其后１５Ｇｙ照射对ＴＣＲ＋，ＣＤ３＋，ＣＤ４＋ＣＤ８＋，ＣＤ４＋ＣＤ８－和ＣＤ４－ＣＤ８＋的损伤作用。表现为各亚组细胞数显著高于单纯１５Ｇｙ照射组。ＣＤ４－ＣＤ８－亚组的细胞数无明显变化。结论００７５ＧｙＸ射线全身照射能够诱导胸腺细胞ＴＣＲ＋，ＣＤ３＋，ＣＤ４＋ＣＤ８＋，ＣＤ４＋ＣＤ８－和ＣＤ４－ＣＤ８＋亚组细胞的适应性反应。Objective\ To study the changes in expression of TCR,CD3 CD4 and CD8 molecules on thymocytes following irradiation with low dose X rays before a challenge dose in mice. Methods Kunming mice were irradiated with 0.075 Gy (conditioning dose) 6 hours before 1.5 Gy irradiation (challenge dose).The expression of TCR,CD3,CD4 and CD8 were analyzed by flow cytometry 18 hours after 1.5 Gy irradiation. Results It was found that the TCR +,CD3 +,CD4 -CD8 +,CD4 +CD8 - and CD4 -CD8 + positive cells were decreased significantly after only 1.5 Gy irradiation.However,the extent of depression of TCR +,CD3 +,CD4 +CD8 +,CD4 +CD8 - and CD4 -CD8 + was diminished when a dose of 0.075 Gy was given 6 hours before 1.5 Gy.The results suggest that pre irradiation with 0.075 X rays can significantly reduce the damage of thymocytes and the matured subsets induced by 1.5 Gy iradiation. Conclusion The results indicate that adaptive response of TCR +,CD3 +,CD4 +CD8 +,CD4 +CD8 - and CD4 -CD8 + subsets of thymocytes can be induced by whole body irradiation with 0.075 Gy X rays.

关 键 词：低剂量辐射 适应性反应 胸腺细胞亚组 

分 类 号：R811.5[医药卫生—放射医学]