α粒子诱导人支气管上皮细胞转化相关基因的分离与克隆  

Isolation and cloning of genes related to the transformation of immortalized human bronchial epithelial cell line induced by alpha particle radiation.

作　　者：周晓[1] 吴德昌[1] 程书钧[1,2] 胡迎春[1] 舒为群[1,2] 李刚[1] 

机构地区：[1]北京放射医学研究所 [2]中国医学科学院肿瘤研究所病因室

出　　处：《中华放射医学与防护杂志》1999年第3期177-179,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助(项目号:39600124);中国博士后科学基金资助

摘　　要：目的探索α粒子照射诱导人支气管上皮细胞转化的相关基因，并加以克隆。方法采用了ｍＲＮＡ差异显示技术，通过辐射诱发转化细胞与同代未经照射细胞的对比，找出差异ｃＤＮＡ片段。结果克隆出２ｇｓ３，５ｇｓ１，５ｇｓ２，２ｇｄ１，６ｇｄ１５个差异片段，测序，经文献检索发现除５ｇｓ１外均为新序列，已登录到ＥＭＢＬ库中。结论发现了细胞转化早期的新序列的４个ｃＤＮＡ片段，生物学特性与功能有待研究。Objective\ To find out the genes related to transformation of human bronchial epithelial cell line and to clone them. Methods The mRNA differential display method was used. Results Differential cDNA fragments were found.After sequencing and identification in GenBank,4 fragments were found to be novel ones and were registered in EMBL database. Conclusion Four novel cDNA fragments have been found,but their biological characterictics and functions are still to be elucidated.

关 键 词：α照射 人支气管上皮细胞 MRNA差异显示 转化相关基因 

分 类 号：X591[环境科学与工程—环境工程]