辐射诱发WI-38细胞遗传不稳定性中的mRNA差异显示分析  

A mRNA DD assay in the studies of genetic instability in WI 38 cells induced by radiation.

作　　者：刘炳辰[1] 吴红英[1] 岳井银[1] 穆传杰[1] 周继文[1] 

机构地区：[1]中国医学科学院中国协和医科大学放射医学研究所

出　　处：《中华放射医学与防护杂志》1999年第3期180-182,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金

摘　　要：目的利用ｍＲＮＡ差异显示技术分析在遗传不稳定性产生和传递过程中，是否有一些特异的基因表达的变化，并在此基础上了解哪些基因在此过程中起关键作用。方法人胚胎成纤维细胞ＷＩ３８经γ射线０５、１０、３０、５０、７０Ｇｙ照射后８小时及３Ｇｙ照射后４、８、２４、４８、１２０小时，提取细胞ＲＮＡ，进行ｍＲＮＡＤＤＰＣＲ，并将差异表达片段进行再扩增。结果ＰＣＲ扩增产物经电泳后，有１４条表达差异的带型出现，所有差异表达片段均获得了再扩增。结论结果说明，某些特异的基因变化参与到了这一过程中，根据我们以前的实验推测，很可能是修复基因的改变。目前正在对这些未知片段进行克隆和序列分析。Objective\ To study the changes in expression of some specific genes in the process of formation and of genetic instability and to determine which genes play a critical role in this process. Methods The total RNA from human fetal fibroblast cell WI 38 was extracted 8 hours after irradiation with 0.5、 1.0、 3.0、 5.0、 7.0 Gy γ rays and at 4, 8, 24, 48, 120 hours after irradiation with 3.0 Gy γ rays. The mRNA DD PCR technique was used to analyze gene expression. Results The gel electrophoresis results showed that 14 differential expression bands appeared as compared with the control.All these differential fragments could be reamplified. Conclusion The results show that some specific genes participate in this proces.They might belong to repair genes according to our results observed before.We are going to clone and sequence these unknown fragments in further studies.

关 键 词：遗传不稳定性 MRNA差异显示 辐射 

分 类 号：R818.03[医药卫生—放射医学]