延缓生长法保存长白山珍稀植物玫瑰种质初探  

Preliminary study through deferring growth on rare plant for germplasm conservation of Rosa rugosa in Changbai Mountain

作　　者：顾地周[1] 高捍东[2] 邱德有[3] 

机构地区：[1]通化师范学院生物系,吉林通化134002 [2]南京林业大学森林资源与环境学院,江苏南京210037 [3]中国林业科学研究院林业研究所,北京100091

出　　处：《中南林业科技大学学报》2010年第7期91-93,104,共4页Journal of Central South University of Forestry & Technology

基　　金：国家科技部"国家科技攻关计划引导项目"(2005BA741C)

摘　　要：以玫瑰嫩茎基部直接再生形成的丛生芽团为试材,应用均匀设计法探讨了影响玫瑰丛生芽团试管保存的主要因子和水平,同时筛选最适于玫瑰丛生芽团试管保存的培养基,结果表明:最适宜的丛生芽团试管保存培养基为B5+根皮苷2.80 mg/L+KT 0.40 mg/L,保存40个月生长率仅为0.83%。对保存前后的芽苗生根的结果进行对比可知,保存后的生根速度快,生根率达99.8%以上,较未保存前的生根率98.5%高。试验结果证明,在试管内采取"延缓生长"法保存玫瑰丛生芽团是切实可行的新方法。In this study,the adventitious buds induced from the tender stems of Rosa rugosa was used as explant,the main factors with different levels for germplasm in vitro conservation were investigated through uniform design experiments,and to screen the appropriate medium for in vitro conservation the adventitious buds of Rosa rugosa.The results showed that B 5＋phloridzin 2.80 mg/L＋KT 0.40 mg/L was suitable for in vitro conservation of the adventitious buds,the rate of regeneration was 0.83 % in 40 months.The contrast test results of rooting of before and after in vitro conservation of the adventitious buds,shoots take root quickly after in vitro conservation,the rate of rooting was more than 99.8 %,which is higher rooting rate than 98.5 % before in vitro conservation.The experiment results proved that in vitro conservation the adventitious buds of Rosa rugosa is feasible new method by deferring growth.

关 键 词：森林培育学 玫瑰 延缓生长 试管保存 根皮苷 均匀设计 

分 类 号：S718.43[农业科学—林学]