开心胶囊对心肌缺血大鼠血清一氧化氮的影响  

Effects of Kaixin Capsule on Nitric Oxide Content in Rats with Myocatdial Ischemia

作　　者：李松[1] 

机构地区：[1]广州中医药大学第一附属医院急症中心,广州510405

出　　处：《广州中医药大学学报》1999年第2期124-126,共3页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：广东省中医药管理局资助!B97-66

摘　　要：为寻找防治无症状心肌缺血的有效中药，以益气养阴名方生脉散加减组成的开心胶囊，对实验性大鼠心肌缺血的预防效果及一氧化氮相关机制进行了研究。将30只大鼠随机分为正常组、缺血组和缺血加灌药组，用硝酸根还原酶（nitratereductase，NR）与葡萄糖-6-磷酸脱氨酶（glucose-6-phosphatedehydroge－nase，GD）结合Gness反应的方法测定其缺血前后大鼠血清一氧化氮浓度（nitricoxide，NO）。结果发现缺血组NO浓度为（2．02±0．19）μmol／L，较正常组（6．55±0．3）μmol／L为低（P＜0．001，N＝10只），而缺血加灌药组以开心胶囊（0．5g／d）灌胃15d后，大鼠血清NO浓度为（4．49±0．90）μmol／L，较缺血组提高（P＜0．01，N＝10只）。提示中药开心胶囊对大鼠心肌缺血有较好的防治作用，其机制可能与提高大鼠血清一氧化氮浓度有关。To find an effective Chinese drug for silent myocardial ischemia(SMI),the preventive ef-fects of Kaixin Capsule(KC),which is composed of modified Shengmai San, on rats with SMI and itsnitric oxide(NO) mechanism were studied.Thirty rats were randomly allocated to three groups: normalgroup(Group Ⅰ,10),SMI group(Group Ⅱ,10) and SMI treated with KC group (Group Ⅲ,10).The serum NO contents were measured by Griess reaction combined with nitrate reductase and glu-cose-6-phosphate dehydrogenase.The results showed that the content of NO in Group Ⅱ (2.02±0.19μmol/L)was markedly decreased as compared with that in Group Ⅰ(6.55±0.37μmol/L)(P<0.001), while it was significantly increased in Group Ⅲ(4.49±0.90μmol/L) as compared with thatin Group Ⅱ(P<0.001).It is indicated that KC is effective for SMI and itS mechanism is possibly re-lated to the increase of serum NO level.

关 键 词：心肌缺血 生脉散 开心胶囊 中医药疗法 

分 类 号：R259.422[医药卫生—中西医结合]