高摄铝下燃煤型氟中毒大鼠肝超微结构观察  

STUDY ON THE LIVER ULTRASTRUCTURAL OF THE BURN-COAL FLUOROSIS IN THE HIGH ALUMINUM RATS

作　　者：黄德远[1] 王丹[2] 张秀慧[2] 喻茂娟[2] 刘鲜林[2] 常傲霜[2] 

机构地区：[1]贵州贵阳市第一人民医院,贵阳550001 [2]贵阳医学院

出　　处：《现代预防医学》2010年第16期3104-3107,共4页Modern Preventive Medicine

基　　金：国家自然科学基金(30460122)

摘　　要：[目的]观察高摄铝状态下燃煤型氟中毒大鼠肝脏超微结构的变化。[方法]将断乳2周的SD纯系大鼠24只(雌雄各半)按体质量随机分为3组,每组8只,即对照组、高氟组、高氟铝组。对照组食用非病区玉米加工的饲料(含氟5.20mg/L,含铝6.80mg/L),饮用普通自来水(含氟0.69mg/L,含铝0.20mg/L)。其余两组则食用以病区玉米为主加工而成的饲料(含氟110.00mg/kg,含铝19.70mg/L),高氟铝组还饮用自来水配制的高铝水(含氟0.70mg/L,含铝90.00mg/L)。实验期为22周。处死前收集大鼠24h尿液用于测定尿氟、铝的水平,处死后取大鼠四肢骨用于测定骨氟,取大鼠肝脏用于在透射电镜下观察肝脏的超微结构变化。[结果](1)骨氟:高氟铝组[(204.07±63.78)mg/kg]、高氟组[(199.90±106.84)mg/kg]的骨氟高于对照组[(30.06±6.11)mg/kg],组间比较差异均有统计学意义(t=0.62、0.53,P﹤0.01)。(2)尿氟:高氟铝组、高氟组的尿氟[(4.52±3.09)、(5.50±0.74)mg/L]均高于对照组[(0.89±0.36)],t=0.23、0.55,P均﹤0.05)。(3)骨铝:对照组的骨铝水平[(3.32±3.02)mg/kg]明显低于高氟铝组[(374.21±56.11)mg/kg]。(4)尿铝:高氟铝组的尿铝水平[(1.14±0.32)mg/L]高于对照组[(0.73±0.11)mg/L],t=1.92,P均﹤0.05]。(5)肝脏的超微结构:高氟组大鼠肝细胞细胞核轻度皱缩、凹陷,异染色质凝聚,边集,少数线粒体空泡化,线粒体嵴消失,内质网轻度扩张;高氟铝组大鼠肝细胞核轻度皱缩、凹陷,形似土豆,细胞内粗面内质网轻度扩张,部分线粒体周围有间隙,线粒体基质电子密度增大,线粒体嵴模糊不清,部分细胞内可见纤维化。[结论]建立了高摄铝状态下燃煤型氟中毒大鼠模型;高氟能引起肝损害,高氟和高铝对肝脏的损伤有协同作用的趋势。[Objective] To observe the rat liver＇s ultrastructural changes of the burn-coal fluorosis combined aluminum. [Methods] The two week-long SD rats were separated into 5 groups bases on the weight,and there were 8 rats in every group,including control group,high F ＋ AL and high F. The control group were fed with non-endemic area corn（It included content of F 5.2 mg /L and Al 6.80 mg /L） ,and normal tap water（the content of the F 0.69 mg /L and Al 0.20 mg /L） . The other two groups feed primarily processes the feed by the burn corn from the endemic area（the content of the F was 110.00 mg /L and Al was 19.70 mg /L） ,they drink the water with high Al（the content of the F was 0.70mg /L and Al was 90.00 mg /L） . The experimental period was 22 weeks. Before executing,we collected the 24 hours urine of rats to determine the F and aluminum. After executing,the content of F in the four bones of the limbs was measured,and the ultrastructural changes in rat liver was observed under the transmission electron microscope. [Results]（1） The bone fluoride in aluminum fluoride group [（204.07 ± 63.78） mg /kg] and high fluoride group [（199.90 ± 106.84） mg /kg] was higher than that in comparison group [（30.06 ± 6.11） mg /kg]（t = 0.62、 0.53,P﹤ 0.01;（2） Urinary fluoride： The urinary fluoride levels in high fluoride Aluminum Group＇s [（4.52 ± 3.09） mg /L] and high fluoride group（5.50 ± 0.74） mg /L were higher [（0.89 ± 0.37） mg /L] than those in control group.（3） Bone aluminum： the bone aluminum levels in comparison group [（3.32 ± 3.02） mg /kg] was significantly lower than that in high aluminum fluoride group [（374.21 ± 56.11） mg /kg];（4） Urine aluminum： The level in high Aluminum fluoride Group about urine [（1.14 ± 0.32） mg /L] was higher than the comparison group [（0.73 0.11） mg /L],t = 1.92,P﹤0.05].（5） The high fluorine group＇s heterochromatin agglomeratesd and assembled at the periphery. Nucleus enve-lope was sunken and wizened;In t

关 键 词：氟 铝 大鼠 肝脏 超微结构 

分 类 号：R595.1[医药卫生—内科学]