手足口病患儿柯萨奇病毒A10型山东地方株VP1区基因特征分析  

The genetic characterization of VP1 region of Coxsackie virus AIO isolated from hand, foot and mouth disease cases in Shandong Province of China

作　　者：杨赫[1] 陶泽新[2] 王海岩[2] 李岩[2] 樊庆莹[1] 冯艺[2] 李忠[2] 王显军[2] 徐爱强[2] 

机构地区：[1]山东大学公共卫生学院流行病与卫生统计学研究所,济南250012 [2]山东省疾病预防控制中心

出　　处：《中华传染病杂志》2010年第7期385-389,共5页Chinese Journal of Infectious Diseases

基　　金：基金项目：国家科技重大专项课题资助项目（2009ZX10004-202）;山东省医药卫生重点项目计划课题资助项目（2007HZ090）;山东省科技发展计划项目（2009GG10002055）

摘　　要：目的了解手足口病（HFMD）患儿标本中分离到的柯萨奇病毒A10型（Coxsackie virus A10，CVA10）山东地方株VP1区基因特征。方法采集2008年至2009年部分HFMD病例临床标本，细胞培养方法分离病毒，提取病毒核酸，RT—PCR扩增病毒VPl区序列，生物信息学方法鉴定其基因型别。选择代表性CVA10毒株，与GenBank中检索到的其他CVAl0毒株进行核苷酸和氨基酸同源性分析，并以VP1部分序列构建亲缘进化树。结果从山东省HFMD患儿的760份临床标本中分离出330株病毒，其中有17株分子定型鉴定为CVA10-CVA10山东地方株彼此间核苷酸和氨基酸的同源性分别在82．3％～100．0％和94．2％～100．0％，与CVAl0原型株（Kowalik株）核苷酸和氨基酸的同源性为75．6％～76．8％和90．2％～93．2％。亲缘进化树显示，CVA10山东地方株分别属于两个不同的基因亚型。结论CVA10也是引起HFMD的病原体之一，山东省存在CVA10两种基因亚型的共循环。Objective To analyze the genetic characterization of VP1 region of Coxsackie virus A10 （CVA10） isolated from clinical specimens of hand, foot and mouth disease （HFMD） patients in Shandong Province. Methods Clinical specimens were collected from some of HFMD patients from 2008 to 2009. The virus was isolated by cell culture. Total RNA was extraeted, and the VP1 genes of the isolates were amplified by reverse transeription-polymerase chain reaction （RT-PCR） and sequenced. The genotypes were identified by molecular typing method and bioinformaties analysis. Homologous comparison and phylogenetic analysis of representative CVA10 strains were performed. Homologous comparison between the Shandong isolates and strains obtained from GenBank were performed and phylogenetie analysis of some representative CVA10 strains were performed. Results Three hundred and thirty viruses strains were isolated from 760 clinical specimens collected from HFMD patients, and 17 of them were identified as CVA10. The homologies of nueleotide and amino acid of the 17 CVA10 strains were 82.3%-100. 0% and 94.2%-100.0%, respectively. Compared with the prototype strain of CVA10 （Kowalik/USA/2003）, the homologies of nucleotide and amino acid were 75. 6%- 76. 8% and 90. 2%- 93. 20%, respectively. Interestingly, Shandong CVA10 strains were clustered into two distinct subgroups in the phylogenetic tree. Conclusions CVA10 is one of the causative agents of HFMD. Two independently circulating subgroups of CVA10 exist in Shandong province.

关 键 词：手足口病 柯萨奇病毒A10型 基因型 序列分析 

分 类 号：R72[医药卫生—儿科]