犬肾传代细胞分离流行性感冒病毒致细胞病变的敏感性  

The sensitivity of cytopathic effect caused by influenza viruses in cultured Madin-Darby canine kidney cells

作　　者：姜晨彦[1] 聂世娇[1] 吕锡宏[1] 高颖阳[1] 居丽雯[1] 

机构地区：[1]复旦大学公共卫生学院流行病学教研室公共卫生安全教育部重点实验室,上海200032

出　　处：《中华传染病杂志》2010年第7期390-392,共3页Chinese Journal of Infectious Diseases

基　　金：基金项目：国家高技术研究发展计划（863计划）资助项目（2007AA022407）;上海市科委项目（09D21906605）

摘　　要：目的了解犬肾传代细胞系（MDCK）细胞对甲型流行性感冒（流感）患者鼻咽拭标本培养1代、2代、3代致细胞病变的敏感性。方法甲型流感患者鼻咽拭标本在MDCK细胞盲传3代培养，倒置显微镜观察每代细胞致细胞病变效应产生情况。结果经胶体金快速检测为甲型流感的279份流感样患者鼻咽拭标本，MDCK培养第1代产生致细胞病变为184份，占65．9％，第2代产生致细胞病变为255份，占91．4％，第3代产生致细胞病变为269份，占96．4％。279份标本的细胞培养液经多重RT-PCR检测鉴定为甲型流感病毒的有271份。结论MDCK分离培养甲型流感患者鼻咽拭标本经盲传3代后，基本可达到95％以上的阳性分离率。Objective To understand the sensitivity of cytopathogenic effect （CPE） in Madin- Darby canine kidney cells （MDCK） that cultured influenza A pharyngeal swab specimens of patients for one,two and three passages. Methods Influenza A pharyngeal swab specimens of patients were inoculated in MDCK for three blind passages. The presence of CPE of every passage was observed by inverted microscope. Results Of the 279 influenza A pharyngeal swab specimens of patients tested by colloidal gold, the presence of CPE in MDCK for one,two and three passages was 65.9% （184/279）, 91.4% （255/279） and 96. 4% （269/279）, respectively. Two hundred and seventy-one of 279 specimens were identified as influenza A by multiplex reverse transcription-polymerase chain reaction （RT-PCR）. Conclusion The positive separation rate can reach more than 95% by inoculating influenza A pharyngeal swab specimens of patients in MDCK for three blind passages.

关 键 词：流感病毒A型 连续传代 肾 细胞系 致细胞病变 病毒 狗 

分 类 号：R51[医药卫生—内科学]