新生隐球菌TUP1△株群体效应分子的筛选鉴定  

Identification of quorum sensing molecular in Cryptococcus neoformans TUP1△ strain

作　　者：朱定衡[1] 车付彬[2] 徐楠[1] 陈江汉[1] 

机构地区：[1]上海长征医院皮肤科,200003 [2]中国人民解放军北京军区北戴河疗养院皮肤科

出　　处：《中华传染病杂志》2010年第7期398-401,共4页Chinese Journal of Infectious Diseases

基　　金：基金项目：国家自然科学基金资助项目（30872263）;上海市浦江人才计划

摘　　要：目的明确新生隐球菌群体感应现象，分离鉴定其群体效应分子。方法观察以不同密度接种的新生隐球菌TUP1△株生长情况，取高密度生长的培养滤过液加入低密度接种TUP1△株的培养基中，观察滤过液促其生长作用，提纯并分析滤过液中的活性物质。结果新生隐球菌TUP1△株具有密度依赖生长特性，只有高于特定密度接种才能形成正常菌落，从高密度生长菌落培养滤过液中分离出一种11肽，可促低密度TUP1△株生长，命名为quorumsen sing peptide1（QSPl）。人工合成该活性物质QSP1，同样可使低密度接种的TUP1△菌株形成正常菌落。结论新生隐球菌存在群体感应现象，其群体效应分子为QSP1。Objective To clarify the quorum sensing and identify the quorum sensing molecular of Cryptococcus neoformans. Methods The growth of Cryptococcus neoformans TUP1△ strain at different inoculum size was observed. The culture filtrate of high cell density TUP1△ strain was collected and plated on medium with low density TUP1△ cell, and the growth of low cell density TUP1△ was recorded. Finally, the active molecule in culture filtrate was purified and identified. Results The growth of Cryptococcus neoformans TUP1 △ strain was density dependent and the colony could be normally formed only above a specific density. The filtrate from a high cell density TUP1△ culture could promote the growth of low cell density TUP1△, and the active molecule in this culture filtrate was identified to be an oligopeptide composed of 11 amino acids pepride, named quorum sensing peptide 1 （QSP1）. QSP1 was artificially synthesized, which could also promote the colony forming of TUP1△ strain inoculated at low cell density. Conclusion Quorum sensing exists in Cryptococcus neoformans, and the quorum sensing molecular is QSP1.

关 键 词：隐球菌 新型 群体感应 信号传导 肽类 

分 类 号：R379[医药卫生—病原生物学]