聚糖纳米微泡超声造影剂的制备及其超声显影效果  

Preparation and evaluation of nanometer-scale bubbles with surfaces of N-palmitoyl chitosan

作　　者：肖云彬[1] 宾建国[1] 李美瑜[1] 谢佳佳[1] 吴爵非[1] 伍巍兰[1] 刘伊丽[1] 蒋刚彪[2] 宾建平[1] 

机构地区：[1]南方医科大学南方医院心内科,广州510515 [2]华南农业大学资源环境学院制药工程系

出　　处：《中华超声影像学杂志》2010年第8期719-722,共4页Chinese Journal of Ultrasonography

基　　金：基金项目：国家863计划项目（2006AA022478）;国家自然科学基金项目（30870722）

摘　　要：目的 制备聚糖纳米微泡超声造影剂,观察其基本特征及动物超声显影效果.方法 高速剪切法制备纳米微泡超声造影剂,光学显微镜和透射电镜观察纳米微泡大小、形态,马尔文粒度分析仪测粒径分布,细胞计数板计数纳米微泡,Zeta电位仪测表面电位,对比超声观察大鼠肾、心脏超声显影效果并测量声强度.结果 纳米微泡呈空心球形结构,分散均匀,形态规则,平均粒径（617±12）nm,浓度（7.2±0.6）×109/ml,Zeta电位（52.9±1.3）mV.24 h、45 d和90d三个时间点浓度、平均粒径和表面电位无明显差异（P＞0.05）.用其可使小鼠肾、心脏超声显影明显增强,最大视频强度分别达（15.6±1.1）GU、（27.3±2.5）GU,可视增强持续时间（10±2）min.结论 聚糖纳米微泡稳定性和显像效果良好,有可能成为可穿过血管内皮间隙的新一代超声造影剂.Objective To develop nanometer-scale bubbles with surfaces of N-palmitoyl chitosan（PLCS） as ultrasound contrast agent and evaluate its characteristics and acoustic effects in vivo. Methods The PLCS nanobubbles were prepared using a cutting technique at differential high-frequency of shear speed. Both optical and transmission electron micrography were performed to determine the nanobubble size and morphology. Concentration, size-distribution and zeta potential of the PLCS nanobubbles were measured by cell counting chamber, Malvern lazer particle analyzer and zeta-sizer at 1-day, 45-day and 90-day. The acoustic effects of the PLCS nanobubbles on myocardium and renal tissue in 6 normal rats were observed using bolus infusion of the nanobubbles intravenously. The maximum video intensity（VI） was measured.Results The PLCS nanobubbles with nice round-shape and uniform site-distribution were demonstrated.The mean diameter,concentration and zeta potential of the PLCS nanobubbles were （617 ± 12） nm, （7.2 ±0.6） × 109/ml and （52.9 ± 1.3）mV at the 1-day,and all of parameters did not change significantly in 45-day and 90-day （ P 〉 0. 05）. A significant contrast-enhancement was noted on myocardium and renal tissue during infusion of the nanobubbles. VI on both tissues was （15.6 ± 1.1）GU and （27.3 ± 2.5）GU,respectively. The visual contrast-enhancement last up to （10 ± 2）min. Conclusions The PLCS nanometerscale bubbles have excellent physical-features and contrast-enhanced ultrasound effects in vivo. It may develop as a novel contrast ultrasound agent which could cross endothelial cell membrances.

关 键 词：超声检查 微气泡 纳米结构 N-软脂酰基壳聚糖 

分 类 号：R445.1[医药卫生—影像医学与核医学]