利奈唑胺与万古霉素对耐甲氧西林金黄色葡萄球菌体外抗菌活性  

Bactericidal activity of linezolid and vancomycin against methicillin-resistant Staphylococcus aureus in vitro

作　　者：周家军[1] 梁蓓蓓[2] 王睿[2] 祝晓光[1] 宋秀杰[2] 蔡芸[2] 白楠[2] 林德峰[2] 蒋志文[1] 董淑英[1] 

机构地区：[1]蚌埠医学院药学系药理学教研室,安徽蚌埠233030 [2]解放军总医院临床药理研究室,北京100853

出　　处：《中国临床药理学杂志》2010年第8期593-596,共4页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(30873127)

摘　　要：目的评价利奈唑胺(噁唑烷酮类抗菌药)、万古霉素(胺基糖苷类抗生素)2种抗菌药物对耐甲氧西林金黄色葡萄球菌(MRSA)的体外抗菌活性。方法用琼脂二倍稀释法,测定抗菌药物的最低抑菌浓度(MIC);用肉汤稀释法,测定抗菌药物的最低杀菌浓度(MBC),绘制杀菌曲线(KCs)。结果利奈唑胺对临床分离的98株MRSA的MIC50为1μg.mL-1,MIC90为2μg.mL-1,MBC50为8μg.mL-1,MBC90为32μg.mL-1,敏感率为100%;万古霉素对临床分离的98株MRSA的MIC50为1μg.mL-1,MIC90为1μg.mL-1,MBC50为8μg.mL-1,MBC90为32μg.mL-1,敏感率为100%。随着抗菌药物浓度的升高,其杀菌时间缩短不甚明显,呈现非浓度依赖性的特点。结论利奈唑胺对MRSA的体外抗菌活性与万古霉素相当,均显示非浓度依赖性的杀菌曲线。Objective To evaluated the in vitro antimicrobial activities of linezolid and vancomycin agents against methicillin -resistant Staphylococcus aureus （MRSA）. Methods The minimal inhibitory concentrations （MICs） of antibacterial agents were determined by the standard two -fold agar dilution method. The minimal bactericidal concentrations （MBCs） antibacterial agents were determined by tube broth dilution method and time -killing curves were obtained. Results The sensitive rates to linezolid and vancomycin against MRSA were both 100%, the MIC50 and MIC90 were 1 μg·mL^-1,1 μg·mL^-1 and 2 μg·mL^-1, 1 μg·mL^-1. 32 μg·mL^-1 respectively. The MBC50 and MBC90 were both 8μg·mL^-1,32μg·mL^-1, respectively. With the increase of drug concentrations, the killing time did not show obvious decline. Conclusion Linezolid and vancomycin have strong antibacterial activity against MRSA, and they have nonconcentration independent characteristic.

关 键 词：利奈唑胺 万古霉素 耐甲氧西林金黄色葡萄球菌 最低抑菌浓度 杀菌曲线 

分 类 号：R978.19[医药卫生—药品] R978.12[医药卫生—药学]