咪唑啉类药物对星形胶质细胞白细胞介素-4分泌的作用  

Effect of imidazoline drugs on the expression of interleukin-4 in astrocytes

作　　者：张红霞[1] 殷为勇[1] 韩钊[1] 郑荣远[2] 

机构地区：[1]温州医学院附属第一医院脑血管科,浙江温州325000 [2]温州医学院附属第一医院神经内科,浙江温州325000

出　　处：《中国临床药理学杂志》2010年第8期622-624,共3页The Chinese Journal of Clinical Pharmacology

基　　金：温州医学院院级硕博科研基金资助项目(2007SB07)

摘　　要：目的观察咪唑啉类药物(咪唑克生,胍丁胺)调节大鼠星形胶质细胞IL-4分泌的影响。方法 10μg.mL-1脂多糖诱导培养后的星形胶质细胞,分为4组:对照组、咪唑克生组、胍丁胺组及地塞米松组。咪唑克生1mmol.L-1;胍丁胺5μmol.L-1;地塞米松1μmol.L-1。于24h收集上层培养液,用EIL-SA法测定4组的星形胶质细胞分泌IL-4水平。结果用咪唑克生、胍丁胺、地塞米松处理后,IL-4分泌均增加。结论咪唑克生、胍丁胺及地塞米松均促进星形胶质细胞Th2细胞因子分泌,使星形胶质细胞分泌的Th1/Th2细胞因子类型转换,起到免疫调节作用。Objective To explore effect of imidazoline drugs （ idazoxan, agmatine ） on rat astrocyte secretion of interleukin - 4 （ IL - 4）. Methods Astrocytes culture induced by 10 μg·mL^-1 lipopolysaccharide were divided to four groups： control group, idazoxan group, agmatine group and dexamethasone group. The dose of idazoxan was 1 mmol · L^ - 1, the dose of agmatine was 5 p, mol · L^ -1 , the dose of dexamethasone was 1 μg·mL^-1. IL -4 of each goup was detect with ELISA methods. Restilts Idazoxan, agmatine and dexamethasonc increased IL- 4 of astrocytes of Th , respectively. Conclusion Idazoxan, agmatine promote secretion 2 cytokines and shift Th1/Th2 cytokines balance to regulate immune responses.

关 键 词：咪唑克生 胍丁胺 白细胞介素-4 

分 类 号：R979.5[医药卫生—药品] R593[医药卫生—药学]