表达重组人红细胞生成素细胞株的高密度、高表达培养  

High Density and High Expression Culture of Recombinant Human Erythropoietin Expressing Cell Line

作　　者：邹钟诚[1] 胡明[1] 

机构地区：[1]沈阳三生制药股份有限公司,110141

出　　处：《中国生化药物杂志》1999年第3期116-118,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的；用生物反应器培养能表达重组人红细胞生成素的工程细胞，使其在到高密度高表达。方法：先将工程细胞在含有５％胎牛血清的ＤＭＥＭ－Ｆ１２培养基中培养，待细胞总数在到２×１０＾８个以上时，接种到５Ｌ生物反应器中，用含有血清的培养基培养７ｄ后转为无血清ＣＨＯ专用培养基，继续培养３０ｄ。在整个培养过程中，采用流加的方式连续培养，根据情况随时测定培养基中葡萄糖浓度，使其保持高于０．５ｇ／Ｌ。Purpose: Bioreactor was used for the culture of recombinant erythropoietin expressing CHO cell line, in order to realize high density and high expression culture. Methods: Expressing cell line was first culured in flasks with DMEM-F12 medium containing 5% serum. When the total number of the cells reached about 2 × 10~8, they were inoculated into the 5 liters bioreactor. After 7 days culture with medium containing serum, and the serum- free medium was utilized to continue the culture for another 30 days. Based on the growth situation, continuous culture was performed by Per fusion method. Glucose concentration was kept above 0. 5 g/L. Lactate and ammonia were also measured at the same time to avoid their accumulation. Sample was taken everyday for the analysis of EPO expression in the harvest medium. When the culture was over, 0. 25 % trypsin was used to digest the carriers, and the total cell number was counted after the cells dropped from the carriers. Results: The cell density reached 6 ×10~6/ml medium, the expression level was about 30 000 IU/ml, and the expressed EPO had a relatively high biological specific activity. Conclusion: Under adequate culture conditions, high density and high expression culture of the recombinant cell line could be realized by using bioreactor.

关 键 词：生物反应器 红细胞生成素 高密度 培养基 

分 类 号：R973.3[医药卫生—药品]