HPLC测定乙酸在控制G-CSF工程菌高密度发酵质量中的应用研究  

Study on the Application of the Determination of Acetic Acid by HPLC in the Quality Control of G-CSF Engineering Bacterium in High-density Culture

作　　者：周俊岭[1] 刁志宏[2] 赵清[1] 叶绿 方向东[2] 王小宁[2] 

机构地区：[1]第一军医大学中心实验室,广州510515 [2]第一军医大学分子免疫研究所

出　　处：《中国生化药物杂志》1999年第3期119-121,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：广东省自然科学基金!(970322)

摘　　要：采用ＨＰＬＣ和ＧＤＡＳ分析测定Ｇ－ＣＳＦ基因工程菌在发酵罐高密度发酵培养过程中菌体产生积累乙酸的含量，分析确定了乙酸含量与产物表达的量化关系，验证了乙酸抑制产物表达的作用。建立了快速测定菌体培养过程中乙酸积累含量检测和动态菌体产物表达量测定的方法。The accumulated content of acetic acid was analyzed by HPLC and the expressed product was determinated by GDAS (Gel Document Analysis System) in the process of high density ferment culture of the G- CSF engineering bacterium. The quantitative relation between the content of acetic acid and the productivity of expressed proteins was established and the restrained effect of acetic acid on the productivity of expressed proteins was proved form the relation. A rapid method in determining the accumulated content of acetic acid and the productivity from the engineering bacterium of the ferment culture was established.

关 键 词：G-CSF工程菌 发酵培养 乙酸 HPLC GDAS 

分 类 号：R392－33[医药卫生—免疫学]