一氧化氮吸入疗法对烟雾吸入性损伤犬心脏能量代谢和病理变化的研究  

Effect of inhaled nitric oxide on cardiac energy metabolism and cardiac pathological change in dogs with smoke inhalation injury

作　　者：齐顺贞[1,2] 杨宗城[1,2] 何保斌 杨建民[1,2] 黎鳌[1,2] 

机构地区：[1]白求恩国际和平医院烧伤整形科 [2]第三军医大学烧伤研究所

出　　处：《中国危重病急救医学》1999年第7期392-394,共3页Chinese Critical Care Medicine

基　　金：全军"九五"攻关课题资助

摘　　要：目的：评价烟雾吸入性损伤犬吸入一氧化氮（ＮＯ）后心脏病理改变与心室肌组织能量代谢的变化。方法：２１只犬随机分为３组，烟雾吸入损伤后，对照组（８只）单纯吸氧（ＦｉＯ２＝０．４５），治疗组（９只）吸氧（ＦｉＯ２＝０．４５）＋０．００４５％ＮＯ，正常组（４只）不致伤，用于病理和组织学对照。实验终点进行心室肌组织学和病理形态学检测。结果：对照组ＡＴＰ含量和能量负荷（ＥＣ）显著低于正常组和治疗组（Ｐ均＜０．０１），对照组ＡＤＰ和ＡＭＰ含量明显低于正常组（Ｐ均＜０．０１），但ＡＤＰ明显高于治疗组（Ｐ＜０．０５）；心脏光镜和电镜病理所见，治疗组病理性改变程度较对照组轻。结论：在烟雾吸入性损伤犬模型中，吸入ＮＯ可不同程度改善心肌组织能量代谢，心脏病理形态改变也有一定程度减轻，表明ＮＯ吸入疗法对改善心脏能量代谢和病理改变有益。Objective :To assess the effect of inhaled nitric oxide (NO) on cardiac pathological change and cardiac energy metabolism after smoke inhalation injury. Methods :21 dogs were divided randomly into three groups.In the normal group (n=4),animals without injury served as pathological controls.After smoke inhalation injury,the control group (n=8) was given oxygen (FiO2=045),the treatment group (n=9) was given oxygen (FiO2=045) mixed with 0004 5% NO.The myocardial histology was observed in various groups at the end of the experiment. Results :In control group,ATP content and energy changes (EC) were significantly lower than normal and treatment groups (all P<001),and the contents of ADP and AMP were also markedly lower than normal group (all P<001),but ADP was significantly higher than treatment gorup (P<005).Optical and electron microscopy showed that the myocardial pathological changes were milder in treatment group compared with control group. Conclusions :In the canine model of smoke inhalation injury,inhaled NO may improve cardiac energy metabolism and attenuate cardiac damage to certain extent.Inhalation of NO appears to be beneficial for the treatment of acute cardiac dysfunction following smoke inhalation injury.

关 键 词：一氧化氮 灼伤 吸入性 病理 心脏 能量代谢 

分 类 号：R541.902[医药卫生—心血管疾病]