日本血吸虫童虫培养细胞超微结构动态变化的研究  

ULTRASTRUCTURAL DYNAMIC CHANGES OF CULTURED CELLSFROM SCHISTOSOMULA SCHISTOSOMA JAPONICUM

作　　者：董惠芬[1] 蒋明森[1] 陈晓蓓[1] 杨明义[1] 李瑛[1] 周述龙[1] 

机构地区：[1]湖北医科大学寄生虫学教研室,血吸虫病研究室

出　　处：《中国寄生虫病防治杂志》1999年第2期140-142,共3页Chinese Journal of Parasitic Disease Control

基　　金：武汉市青年科技晨光计划;湖北省教委和卫生厅资助

摘　　要：随着培养时间的延长，日本血吸虫童虫培养细胞的超微结构发生一系列渐进性变化，主要表现在：核中异染色质含量逐渐增加，由０ｄ时的显著少于常染质转变为５４ｄ时的高于常染色质；线粒体逐渐变性，嵴由清晰变为模糊以至消失，基质电子密度降低，最终成为空泡；内质网由环状变为短管状、囊泡状，最后消失。日本血吸虫童虫培养细胞中线粒体和内质网超微结构的这些变化，可以用来判断培养条件的优劣；而从染色质的一系列变化推断，０～１８ｄ是诱导培养细胞发生增殖的最佳时期。Ultrastructures of cultured cells from schistosomula Schistosoma japonicum changed progressively along with the prolongation of culture time. Heterochromatin in the nucleus increased, e.g., it was less in the first day and more in the fiftyfourth day than euchromatin. Mitochondria degenerated gradually. Cristae blurred little by little and disappeared after 30 days. Electron density of matrix became lower and lower. At last, mitochondria became vacuolar completely. Endoplasmic reticula changed from circular arrangement into short tubes and vacuoels. They disappeared finally. Presumably, the ultrastructural changes of mitochondria and endoplasmic reticula could be used to evaluate whether the culture conditions were appropriate. And the changes of chromatin indicated that 018 days were the best time for the inducement of cell proliferation.

关 键 词：日本血吸虫 童虫 培养细胞 超微结构 动态变化 

分 类 号：R383.24[医药卫生—医学寄生虫学]