芬氟拉明合用电针对大鼠脑内中央灰质单胺类释放的影响<英文>  

Effects of fenfluramine combined with electroacupuncture on monoamine release in periaqueductal gray of rat brain

作　　者：李晓艳[1] 朱崇斌[1] 陈海南 朱燕华[1] 吴根诚[1] 许绍芬[1] 

机构地区：[1]上海医科大学神经生物学教研室医学神生物学国家重点实验室,上海中国200032

出　　处：《中国药理学报》1999年第7期597-600,共4页Acta Pharmacologica Sinica

基　　金：Project supported by the National Natural Science Foundation of China, № 39670901;by Shanghai Municipal Health Bureau,№ 95(A)1117.

摘　　要：目的:研究5-羟色胺释放剂芬氟拉明加强针刺镇痛前后大鼠脑内中央灰质(PAG)腹侧部单胺类递质的变化。方法:运用微透析及高效液相电化学检测方法。结果:电针后Nor的释放减少,而5-HT,5-HIAA和DA,HVA在AG部位含量升高(P<0.05,vs NS组)。当芬氟拉明合用电针时,5-HT,5-HIAA含量进一步升高,但Nor,DA及其代谢产物却无明显变化(P<0.05 vs NS+EA组)。结论:电针能促进DA和5-HT释放但抑制Nor释放。芬氟拉明合用电针能进一步加强5-HT的释放。芬氟拉明加强针刺镇痛可能与进一步激活5-羟色胺系统有关。AIM: To study the changes of monoamines in ventrolatoral periaqueductal gray of rat brain before and after electroacupuncture (EA) analgesia (EAA) was enhanced by fenfluramine (Fen), a 5-hydroxytrypta-mine (5-HT) releaser. METHODS: Monoamines were collected by in vivo microdialysis and measured by HPLC connected with electrochemical detector. RESULTS: The level of norepinephrine (Nor) after EA was decreased ( P < 0.05 vs NS group). The contents of 5-HT, 5-hydroxyindol acetic acid (5-HIAA), dopamine (DA), and homovanillic acid (HVA) in periaqueductal gray dialysate were increased (P<0.05 vs NS group). When Fen was combined with EA, the level of 5-HT and 5-HLAA were further increased (P < 0.05 vs NS + EA group). There was no obvious change of Nor, DA, and HVA. CONCLUSION: Fen potentiating EAA may be related to further activation of serotoninergic system.

关 键 词：芬氟拉明 灰质 单胺释放 电针 微透析 电化学 

分 类 号：R971.1[医药卫生—药品] R246.2[医药卫生—药学]