侧柏叶HPLC指纹图谱的方法学研究  

HPLC fingerprint analysis of Cacumen Platycladi

作　　者：刘翠玲[1] 魏刚[1] 刘东辉[1] 黄月纯[2] 

机构地区：[1]广州中医药大学,广东广州510405 [2]广州中医药大学第一附属医院,广东广州510405

出　　处：《中成药》2010年第8期1281-1283,共3页Chinese Traditional Patent Medicine

基　　金："十一五"国家支撑计划项目"中医外治特色疗法和外治技术示范研究"(2008BAI53B074)

摘　　要：目的:建立侧柏叶的指纹图谱分析方法,评价侧柏叶药材质量,同时为双柏制剂质量控制提供参考。方法:采用HPLC法测定,色谱柱为ZORBAX Eclipse XDB-C18柱;流动相为乙腈-0.1%磷酸(梯度洗脱);检测波长为254nm;流速为1mL/min;柱温为40℃。结果:14批侧柏叶共标示出11个共有峰与对照品比较,共有峰中检出槲皮苷和槲皮素。结论:方法准确可靠,重复性好,为侧柏叶药材及其制剂质量控制提供了依据。AIM： To establish a method of fingerprint analysis on Cacumen Platycladi. METHODS： HPLC was adopted with ZORBAX Eclipse XDB-C18,acetonitrile-0. 1% H3PO4 solution （gradient alution） as a mobile phase and detection wavelength at 254 nm,flow rate was 1 mL/min,and colum temperature was at 40 ℃. RESULTS： Eleven common peaks were separated from 14 batches of Cacumen Platycladi. As compared with standard sample,quecitroside and quecitin were checked out. CONCLUSION： The method is reliable,accurate and can be used as a quality control method for Cacumen Platycladi.

关 键 词：侧柏叶 指纹图谱 HPLC 

分 类 号：R284.1[医药卫生—中药学]