粉防己碱对LPS诱导的大鼠发热模型的解热作用  

Antifebrile effect of tetrandrine on LPS-induced febris response in rats

作　　者：罗福玲[1] 赵恒光[1] 章卓 李洪忠[3] 万敬员[3] 周岐新[3] 

机构地区：[1]重庆医科大学附属第一医院,重庆400016 [2]泸洲医学院,四川泸州646000 [3]重庆医科大学重庆市生物化学与分子药理学重点实验室,重庆400016

出　　处：《中成药》2010年第8期1304-1308,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(30500463)

摘　　要：目的:研究粉防己碱(tetrandrine,Tet)对脂多糖(lipopolysacchride,LPS)诱导的雄性Sprague-Dawley(SD)大鼠发热模型的解热作用及其机制。方法:48只雄性SD大鼠随机分为空白对照组、LPS模型组、对乙酰氨基酚对照组及粉防己碱5,15,45mg/kg组。用LPS100μg/kg腹腔注射建立大鼠发热模型,药物处理后每30min测体温一次,连续监测6h,观察大鼠的体温变化;运用Western-blot检测脑组织中环加氧酶2(Cyclooxygenase-2,COX-2)的表达,并用EIA法检测其前列腺素E2(Prostaglandin E2,PGE2)的含量;髓过氧化物酶(Myeloperoxidase,MPO)试剂盒检测肝组织MPO水平。结果:粉防己碱能有效抑制LPS诱导的大鼠发热反应,显著降低脑组织中COX-2和PGE2的表达,抑制肝脏MPO水平。结论:粉防己碱具有显著的解热作用,其作用机制可能与抑制脑组织中COX-2和PGE2的表达,降低肝组织中MPO含量有关。AIM： To observe the effect of tetrandrine on LPS-induced febrile response in sprague-dawley（SD） rats and elucidate the antipyretic mechanism of tetrandrine. METHODS： Forty eight male SD rats were randomly assigned to 6 groups： tetrandrine 5,15,45 mg/kg group,model group,normal group,acetaminophen 50 mg/kg positive control group. Fever was induced by intraperitoneally injecting LPS （100 μg/kg）,and the temperature was observed every 30 minutes,the observation lasted for 6 h,we observed the changes of temperature. Western blotting technologies and euzyme-linked immunosorbent assays were applied to the expression of cyclooxygenase-2 （COX-2） and Prostaglandin E2（PGE2） in rat brain tissues,and myeloperoxidase（MPO） in liver was tested by special kits. RESULTS： Tetrandrine could effectively decrease febrile response,inhibit the expression of COX-2 and PGE2 in brain,lower liver MPO level in LPS-induced fever rats. CONCLUSION： Tetrandrine has the action of antipyretic effect. Its mechanism may be related to inhibition of the expression of COX-2 and PGE2 in brain tissues,and level of MPO in liver.

关 键 词：粉防己碱 脂多糖 发热 环氧化物酶2 前列腺素E 大鼠 

分 类 号：R966[医药卫生—药理学]