制川乌配伍白芍微波提取与传统提取工艺的比较研究  

Comparison between microwave and reflux extraction of Radix Aconiti praeparata coupled with Radix Paeoniae alba

作　　者：郑琴[1] 郝伟伟[1] 乐志艳[1] 陆浩伟[1] 卢燕香[1] 杨明[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《中成药》2010年第8期1320-1324,共5页Chinese Traditional Patent Medicine

基　　金：江西省教育厅科学技术研究项目(GJJ08333)

摘　　要：目的:比较微波提取与传统工艺对制川乌和白芍中有效成分乌头总生物碱、芍药苷及浸膏得率的影响研究。方法:以L9(34)正交设计法,用紫外可见分光光度法测定乌头总生物碱,采用HPLC法测定芍药苷,并以其含量及浸膏得率作为评价指标。结果:优化微波提取工艺为:微波辐射功率800W,微波辐射时间为30min,乙醇浓度为65%,料液比为1∶10;传统提取工艺为:乙醇浓度为65%,物料比1∶10,提取时间为2h,提取次数2次。结论:微波提取时间短,有效成分提取率高。AIM： To compare the extraction rate of bioactive components and yield of the extraction from Radix Aconiti praeparata coupled with Radix Paeoniae alba drug-pair between microwave extraction （ME） with reflux extraction,the following study was carried out. METHODS： Orthogonal test,ME,UV-spect-rophotometry and HPLC were employed in the experiment. The contents of total alkaloids in Aconiti preparata and the paeoniflorin in Paeoniae alba were measured besides the yield of extraction. RESULTS： The optimum condition of the microwave extraction was that the solvent was 65% ethanol,the microwave power was 800 W,the proportion of raw material to solvent was 1 ∶ 10 and the radiation time was 30 min. The optimum reflux extraction was that 10 times of 65% ethanol and extracted for 2 times,boiling range for 2 h. CONCLUSION： The optimal microwave extraction technology can provide a high yield and need less time.

关 键 词：药对 微波提取 回流提取 正交设计 

分 类 号：R284.2[医药卫生—中药学]